Mouse CA12/Carbonic anhydrase 12 ELISA Kit PicoKine®

Carbonic anhydrase 12 is an enzyme that in humans is encoded by the CA12 gene. It is mapped to 9; 9 C. This gene encodes a membrane-bound member of the alpha carbonic anhydrase family of enzymes that catalyze the reversible hydration of carbon dioxide to bicarbonate. These proteins participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Alternative splicing results in multiple transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 51 39 35 41 1.41 3.5% Sample 2 184 150 174 167 169 11.04 6.5% Sample 3 567 804 798 811 745 41.79 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 51 39 35 41 1.41 3.5% Sample 1 37 51 39 35 41 1.41 3.5% Sample 2 184 150 174 167 169 11.04 6.5% Sample 2 184 150 174 167 169 11.04 6.5% Sample 3 567 804 798 811 745 41.79 5.6% Sample 3 567 804 798 811 745 41.79 5.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 184 567 44 179 642 Standard deviation 2.40 9.86 11.79 3.49 11.31 16.24 CV (%) 6.5% 5.4% 2.1% 7.9% 6.3% 2.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 184 567 44 179 642 Standard deviation 2.40 9.86 11.79 3.49 11.31 16.24 CV (%) 6.5% 5.4% 2.1% 7.9% 6.3% 2.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 37 184 567 44 179 642 Mean (pg/ml) 37 184 567 44 179 642 Standard deviation 2.40 9.86 11.79 3.49 11.31 16.24 Standard deviation 2.40 9.86 11.79 3.49 11.31 16.24 CV (%) 6.5% 5.4% 2.1% 7.9% 6.3% 2.5% CV (%) 6.5% 5.4% 2.1% 7.9% 6.3% 2.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.048 0.12 0.179 0.289 0.455 0.818 1.321 2.396 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.048 0.12 0.179 0.289 0.455 0.818 1.321 2.396 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.048 0.12 0.179 0.289 0.455 0.818 1.321 2.396 O.D. 0.048 0.12 0.179 0.289 0.455 0.818 1.321 2.396

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.