Mouse CA12/Carbonic anhydrase 12 ELISA Kit PicoKine®

Mouse CA12 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Ca12 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Carbonic anhydrase 12 is an enzyme that in humans is encoded by the CA12 gene. It is mapped to 9; 9 C. This gene encodes a membrane-bound member of the alpha carbonic anhydrase family of enzymes that catalyze the reversible hydration of carbon dioxide to bicarbonate. These proteins participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Alternative splicing results in multiple transcript variants.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 51 39 35 41 1.41 3.5% Sample 2 184 150 174 167 169 11.04 6.5% Sample 3 567 804 798 811 745 41.79 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 51 39 35 41 1.41 3.5% Sample 1 37 51 39 35 41 1.41 3.5% Sample 2 184 150 174 167 169 11.04 6.5% Sample 2 184 150 174 167 169 11.04 6.5% Sample 3 567 804 798 811 745 41.79 5.6% Sample 3 567 804 798 811 745 41.79 5.6%

Synonyms

Carbonic anhydrase 12; Carbonate dehydratase XII; Carbonic anhydrase XII; CA-XII; Ca12; Car12

Gene Name

Ca12

Gene ID

76459

UniProt

Q8CI85

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A25-S301

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

31.2 pg/mL -2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 184 567 44 179 642 Standard deviation 2.40 9.86 11.79 3.49 11.31 16.24 CV (%) 6.5% 5.4% 2.1% 7.9% 6.3% 2.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 184 567 44 179 642 Standard deviation 2.40 9.86 11.79 3.49 11.31 16.24 CV (%) 6.5% 5.4% 2.1% 7.9% 6.3% 2.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 37 184 567 44 179 642 Mean (pg/ml) 37 184 567 44 179 642 Standard deviation 2.40 9.86 11.79 3.49 11.31 16.24 Standard deviation 2.40 9.86 11.79 3.49 11.31 16.24 CV (%) 6.5% 5.4% 2.1% 7.9% 6.3% 2.5% CV (%) 6.5% 5.4% 2.1% 7.9% 6.3% 2.5%

Sensitivity

<10 pg/mL

Function

Reversible hydration of carbon dioxide.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse CA12

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Carbonic anhydrase 12

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.048 0.12 0.179 0.289 0.455 0.818 1.321 2.396 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.048 0.12 0.179 0.289 0.455 0.818 1.321 2.396 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.048 0.12 0.179 0.289 0.455 0.818 1.321 2.396 O.D. 0.048 0.12 0.179 0.289 0.455 0.818 1.321 2.396

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.