Rat SEZ6L ELISA Kit PicoKine®

Rat SEZ6L ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Sez6l in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Seizure 6-like protein is a protein that in humans is encoded by the SEZ6L gene. It is mapped to 12q16.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 176 199 178 190 185 9.33 5.0% Sample 2 807 825 758 743 783 33.78 4.3% Sample 3 4126 3875 3712 3928 3910 147.82 3.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 176 199 178 190 185 9.33 5.0% Sample 1 176 199 178 190 185 9.33 5.0% Sample 2 807 825 758 743 783 33.78 4.3% Sample 2 807 825 758 743 783 33.78 4.3% Sample 3 4126 3875 3712 3928 3910 147.82 3.7% Sample 3 4126 3875 3712 3928 3910 147.82 3.7%

Synonyms

Seizure-related 6 homolog-like; Sez6l

Gene Name

Sez6l

Gene ID

304554

UniProt

D4AD89

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L29-A958

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 176 807 4126 260 941 4859 Standard deviation 8.62 34.70 169.17 15.60 54.58 335.27 CV (%) 4.9% 4.3% 4.1% 6.0% 5.8% 6.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 176 807 4126 260 941 4859 Standard deviation 8.62 34.70 169.17 15.60 54.58 335.27 CV (%) 4.9% 4.3% 4.1% 6.0% 5.8% 6.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 176 807 4126 260 941 4859 Mean (pg/ml) 176 807 4126 260 941 4859 Standard deviation 8.62 34.70 169.17 15.60 54.58 335.27 Standard deviation 8.62 34.70 169.17 15.60 54.58 335.27 CV (%) 4.9% 4.3% 4.1% 6.0% 5.8% 6.9% CV (%) 4.9% 4.3% 4.1% 6.0% 5.8% 6.9%

Sensitivity

<50 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant rat SEZ6L

Anticoagulant

Heparin

Gene Name Synonym

Seizure related 6 homolog like

Subcellular Location

Endoplasmic reticulum. Integral component of membrane. Neuronal cell body.

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.054 0.155 0.238 0.366 0.646 1.028 1.638 2.224 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.054 0.155 0.238 0.366 0.646 1.028 1.638 2.224 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.054 0.155 0.238 0.366 0.646 1.028 1.638 2.224 O.D. 0.054 0.155 0.238 0.366 0.646 1.028 1.638 2.224

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.