Human CD155/PVR/Poliovirus receptor ELISA Kit PicoKine®

CD155 (cluster of differentiation 155) also known as the poliovirus receptor is a protein that in humans is encoded by the PVR gene. It is mapped to 19q13.31. The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 187 172 165 194 179 11.54 6.4% Sample 2 714 642 653 620 657 34.85 5.3% Sample 3 4324 4263 4182 4377 4286 72.57 1.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 187 172 165 194 179 11.54 6.4% Sample 1 187 172 165 194 179 11.54 6.4% Sample 2 714 642 653 620 657 34.85 5.3% Sample 2 714 642 653 620 657 34.85 5.3% Sample 3 4324 4263 4182 4377 4286 72.57 1.6% Sample 3 4324 4263 4182 4377 4286 72.57 1.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 187 714 4324 212 881 4527 Standard deviation 8.98 34.99 198.90 11.87 60.79 289.73 CV (%) 4.8% 4.9% 4.6% 5.6% 6.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 187 714 4324 212 881 4527 Standard deviation 8.98 34.99 198.90 11.87 60.79 289.73 CV (%) 4.8% 4.9% 4.6% 5.6% 6.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 187 714 4324 212 881 4527 Mean (pg/ml) 187 714 4324 212 881 4527 Standard deviation 8.98 34.99 198.90 11.87 60.79 289.73 Standard deviation 8.98 34.99 198.90 11.87 60.79 289.73 CV (%) 4.8% 4.9% 4.6% 5.6% 6.9% 6.4% CV (%) 4.8% 4.9% 4.6% 5.6% 6.9% 6.4%

Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. (Microbial infection) Acts as a receptor for poliovirus. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport. (Microbial infection) Acts as a receptor for Pseudorabies virus. (Microbial infection) Is prevented to reach cell surface upon infection by Human cytomegalovirus /HHV-5, presumably to escape immune recognition of infected cell by NK cells.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.022 0.169 0.275 0.445 0.771 1.173 1.451 1.939 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.022 0.169 0.275 0.445 0.771 1.173 1.451 1.939 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.022 0.169 0.275 0.445 0.771 1.173 1.451 1.939 O.D. 0.022 0.169 0.275 0.445 0.771 1.173 1.451 1.939

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.