Rat EphA4 ELISA Kit PicoKine®

EPH receptor A4 (ephrin type-A receptor 4) is a protein that in humans is encoded by the EPHA4 gene. It is mapped to 9q33. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 47 55 52 55 52 3.26 6.2% Sample 2 150 132 148 145 143 7.01 4.9% Sample 3 534 536 553 568 547 13.82 2.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 47 55 52 55 52 3.26 6.2% Sample 1 47 55 52 55 52 3.26 6.2% Sample 2 150 132 148 145 143 7.01 4.9% Sample 2 150 132 148 145 143 7.01 4.9% Sample 3 534 536 553 568 547 13.82 2.5% Sample 3 534 536 553 568 547 13.82 2.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 47 150 534 56 224 767 Standard deviation 2.77 6.45 21.89 3.70 12.99 58.29 CV (%) 5.9% 4.3% 4.1% 6.6% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 47 150 534 56 224 767 Standard deviation 2.77 6.45 21.89 3.70 12.99 58.29 CV (%) 5.9% 4.3% 4.1% 6.6% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 47 150 534 56 224 767 Mean (pg/ml) 47 150 534 56 224 767 Standard deviation 2.77 6.45 21.89 3.70 12.99 58.29 Standard deviation 2.77 6.45 21.89 3.70 12.99 58.29 CV (%) 5.9% 4.3% 4.1% 6.6% 5.8% 7.6% CV (%) 5.9% 4.3% 4.1% 6.6% 5.8% 7.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.02 0.097 0.154 0.259 0.472 0.837 1.395 2.314 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.02 0.097 0.154 0.259 0.472 0.837 1.395 2.314 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.02 0.097 0.154 0.259 0.472 0.837 1.395 2.314 O.D. 0.02 0.097 0.154 0.259 0.472 0.837 1.395 2.314

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.