Mouse Contactin-6 ELISA Kit PicoKine®

Contactin 6 is a protein in humans that is encoded by the CNTN6 gene. It is mapped to 6; 6 E1. The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI) -anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 199 179 195 198 192 8.07 4.2% Sample 2 865 896 789 851 850 38.93 4.5% Sample 3 4171 3634 3979 3692 3869 217.86 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 199 179 195 198 192 8.07 4.2% Sample 1 199 179 195 198 192 8.07 4.2% Sample 2 865 896 789 851 850 38.93 4.5% Sample 2 865 896 789 851 850 38.93 4.5% Sample 3 4171 3634 3979 3692 3869 217.86 5.6% Sample 3 4171 3634 3979 3692 3869 217.86 5.6%

Expressed in brain. In brain, it is preferentially expressed in the accessory olfactory bulb, layers II/III and V of the cerebral cortex, piriform cortex, anterior thalamic nuclei, locus coeruleus of the pons and mesencephalic trigeminal nucleus and in Purkinje cells of the cerebellum.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 199 865 4171 224 965 4287 Standard deviation 9.55 37.20 171.01 14.78 55.97 325.81 CV (%) 4.8% 4.3% 4.1% 6.6% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 199 865 4171 224 965 4287 Standard deviation 9.55 37.20 171.01 14.78 55.97 325.81 CV (%) 4.8% 4.3% 4.1% 6.6% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 199 865 4171 224 965 4287 Mean (pg/ml) 199 865 4171 224 965 4287 Standard deviation 9.55 37.20 171.01 14.78 55.97 325.81 Standard deviation 9.55 37.20 171.01 14.78 55.97 325.81 CV (%) 4.8% 4.3% 4.1% 6.6% 5.8% 7.6% CV (%) 4.8% 4.3% 4.1% 6.6% 5.8% 7.6%

Contactins mediate cell surface interactions during nervous system development. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus (By similarity) . Involved in motor coordination.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.061 0.117 0.223 0.385 0.822 1.786 1.979 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.061 0.117 0.223 0.385 0.822 1.786 1.979 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.061 0.117 0.223 0.385 0.822 1.786 1.979 O.D. 0.025 0.061 0.117 0.223 0.385 0.822 1.786 1.979

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.