Human Contactin-5 ELISA Kit PicoKine®

Contactin-5 is a protein that in humans is encoded by the CNTN5 gene. It is mapped to 11q22.1. The protein encoded by this gene is a member of the immunoglobulin superfamily, and contactin family, which mediate cell surface interactions during nervous system development. This protein is a glycosylphosphatidylinositol (GPI) -anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 302 291 294 288 294 12.34 4.2% Sample 2 1651 1636 1744 1596 1657 125.91 7.6% Sample 3 6356 5997 6413 6982 6437 321.85 5.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 302 291 294 288 294 12.34 4.2% Sample 1 302 291 294 288 294 12.34 4.2% Sample 2 1651 1636 1744 1596 1657 125.91 7.6% Sample 2 1651 1636 1744 1596 1657 125.91 7.6% Sample 3 6356 5997 6413 6982 6437 321.85 5.0% Sample 3 6356 5997 6413 6982 6437 321.85 5.0%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 302 1651 6356 294 1742 6883 Standard deviation 12.08 94.11 292.38 14.99 122.2 481.81 CV (%) 4.0% 5.7% 4.6% 5.1% 6.9% 7.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 302 1651 6356 294 1742 6883 Standard deviation 12.08 94.11 292.38 14.99 122.2 481.81 CV (%) 4.0% 5.7% 4.6% 5.1% 6.9% 7.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 302 1651 6356 294 1742 6883 Mean (pg/ml) 302 1651 6356 294 1742 6883 Standard deviation 12.08 94.11 292.38 14.99 122.2 481.81 Standard deviation 12.08 94.11 292.38 14.99 122.2 481.81 CV (%) 4.0% 5.7% 4.6% 5.1% 6.9% 7.0% CV (%) 4.0% 5.7% 4.6% 5.1% 6.9% 7.0%

Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity in the cerebral cortical neurons but not in hippocampal neurons. Probably involved in neuronal activity in the auditory system.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expressed in brain and kidney and at very low level in placenta. Not expressed in other tissues. In brain, it is highly expressed in the occipital lobe, amygdala, cerebral cortex, frontal lobe, thalamus and temporal lobe. Expressed at moderate level in the cerebellum, substantia nigra, putamen, medulla and hippocampus. Weakly expressed in the spinal cord and caudate nucleus. Weakly or not expressed in the corpus callosum.

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.046 0.096 0.138 0.244 0.422 0.843 1.607 2.646 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.046 0.096 0.138 0.244 0.422 0.843 1.607 2.646 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.046 0.096 0.138 0.244 0.422 0.843 1.607 2.646 O.D. 0.046 0.096 0.138 0.244 0.422 0.843 1.607 2.646

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.