Mouse Contactin-4 ELISA Kit PicoKine®

Contactin-4 is a protein that in humans is encoded by the CNTN4 gene. It is mapped to 6; 6 E1. The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI) -anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 451 511 512 479 488 25.27 5.1% Sample 2 1849 2120 1840 2095 1976 131.83 6.6% Sample 3 7615 7780 8028 8212 7908 228.60 2.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 451 511 512 479 488 25.27 5.1% Sample 1 451 511 512 479 488 25.27 5.1% Sample 2 1849 2120 1840 2095 1976 131.83 6.6% Sample 2 1849 2120 1840 2095 1976 131.83 6.6% Sample 3 7615 7780 8028 8212 7908 228.60 2.8% Sample 3 7615 7780 8028 8212 7908 228.60 2.8%

Expressed in the region of developing olfactory neurons. Isoform 2 is expressed in mature sensory cells of the vomeronasal neuroepithelium and at lower level in olfactory neuroepithelium.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 451 1849 7615 513 2075 8739 Standard deviation 26.61 79.51 312.22 33.86 118.28 681.64 CV (%) 5.9% 4.3% 4.1% 6.6% 5.7% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 451 1849 7615 513 2075 8739 Standard deviation 26.61 79.51 312.22 33.86 118.28 681.64 CV (%) 5.9% 4.3% 4.1% 6.6% 5.7% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 451 1849 7615 513 2075 8739 Mean (pg/ml) 451 1849 7615 513 2075 8739 Standard deviation 26.61 79.51 312.22 33.86 118.28 681.64 Standard deviation 26.61 79.51 312.22 33.86 118.28 681.64 CV (%) 5.9% 4.3% 4.1% 6.6% 5.7% 7.8% CV (%) 5.9% 4.3% 4.1% 6.6% 5.7% 7.8%

Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity. May be involved in synaptogenesis.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.053 0.138 0.192 0.321 0.509 0.902 1.476 1.95 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.053 0.138 0.192 0.321 0.509 0.902 1.476 1.95 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.053 0.138 0.192 0.321 0.509 0.902 1.476 1.95 O.D. 0.053 0.138 0.192 0.321 0.509 0.902 1.476 1.95

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.