Human Contactin-4 ELISA Kit PicoKine®

Contactin-4 is a protein that in humans is encoded by the CNTN4 gene. It is mapped to 3p26.3-p26.2. This gene encodes a member of the contactin family of immunoglobulins. Contactins are axon-associated cell adhesion molecules that function in neuronal network formation and plasticity. The encoded protein is a glycosylphosphatidylinositol-anchored neuronal membrane protein that may play a role in the formation of axon connections in the developing nervous system. Deletion or mutation of this gene may play a role in 3p deletion syndrome and autism spectrum disorders. Alternative splicing results in multiple transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 343 376 320 370 352 22.38 6.3% Sample 2 1884 1754 1627 1875 1785 104.67 5.8% Sample 3 8803 9355 8126 9099 8845 459.16 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 343 376 320 370 352 22.38 6.3% Sample 1 343 376 320 370 352 22.38 6.3% Sample 2 1884 1754 1627 1875 1785 104.67 5.8% Sample 2 1884 1754 1627 1875 1785 104.67 5.8% Sample 3 8803 9355 8126 9099 8845 459.16 5.1% Sample 3 8803 9355 8126 9099 8845 459.16 5.1%

Mainly expressed in brain. Highly expressed in cerebellum and weakly expressed in corpus callosum, caudate nucleus, amygdala and spinal cord. Also expressed in testis, pancreas, thyroid, uterus, small intestine and kidney. Not expressed in skeletal muscle. Isoform 2 is weakly expressed in cerebral cortex.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 343 1884 8803 425 2121 9479 Standard deviation 14.75 97.97 404.94 25.93 125.14 701.45 CV (%) 4.3% 5.2% 4.6% 6.1% 5.9% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 343 1884 8803 425 2121 9479 Standard deviation 14.75 97.97 404.94 25.93 125.14 701.45 CV (%) 4.3% 5.2% 4.6% 6.1% 5.9% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 343 1884 8803 425 2121 9479 Mean (pg/ml) 343 1884 8803 425 2121 9479 Standard deviation 14.75 97.97 404.94 25.93 125.14 701.45 Standard deviation 14.75 97.97 404.94 25.93 125.14 701.45 CV (%) 4.3% 5.2% 4.6% 6.1% 5.9% 7.4% CV (%) 4.3% 5.2% 4.6% 6.1% 5.9% 7.4%

Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity. May be involved in synaptogenesis.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.049 0.126 0.193 0.285 0.488 0.874 1.516 2.272 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.049 0.126 0.193 0.285 0.488 0.874 1.516 2.272 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.049 0.126 0.193 0.285 0.488 0.874 1.516 2.272 O.D. 0.049 0.126 0.193 0.285 0.488 0.874 1.516 2.272

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.