Human Contactin-2 ELISA Kit PicoKine®

Contactin-2 is a protein that in humans is encoded by the CNTN2 gene. It is mapped to 1q32.1. This gene encodes a member of the contactin family of proteins, part of the immunoglobulin superfamily of cell adhesion molecules. The encoded glycosylphosphatidylinositol (GPI) -anchored neuronal membrane protein plays a role in the proliferation, migration, and axon guidance of neurons of the developing cerebellum. A mutation in this gene may be associated with adult myoclonic epilepsy.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 79 77 84 76 79 3.08 3.8% Sample 2 331 351 334 321 334 10.80 3.2% Sample 3 872 1022 929 988 952 57.27 6.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 79 77 84 76 79 3.08 3.8% Sample 1 79 77 84 76 79 3.08 3.8% Sample 2 331 351 334 321 334 10.80 3.2% Sample 2 331 351 334 321 334 10.80 3.2% Sample 3 872 1022 929 988 952 57.27 6.0% Sample 3 872 1022 929 988 952 57.27 6.0%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 79 331 872 82 352 939 Standard deviation 3.87 14.23 35.75 5.41 20.42 71.36 CV (%) 4.9% 4.3% 4.1% 6.6% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 79 331 872 82 352 939 Standard deviation 3.87 14.23 35.75 5.41 20.42 71.36 CV (%) 4.9% 4.3% 4.1% 6.6% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 79 331 872 82 352 939 Mean (pg/ml) 79 331 872 82 352 939 Standard deviation 3.87 14.23 35.75 5.41 20.42 71.36 Standard deviation 3.87 14.23 35.75 5.41 20.42 71.36 CV (%) 4.9% 4.3% 4.1% 6.6% 5.8% 7.6% CV (%) 4.9% 4.3% 4.1% 6.6% 5.8% 7.6%

In conjunction with another transmembrane protein, CNTNAP2, contributes to the organization of axonal domains at nodes of Ranvier by maintaining voltage-gated potassium channels at the juxtaparanodal region. May be involved in cell adhesion.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.007 0.035 0.079 0.172 0.364 0.799 1.518 2.021 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.007 0.035 0.079 0.172 0.364 0.799 1.518 2.021 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.007 0.035 0.079 0.172 0.364 0.799 1.518 2.021 O.D. 0.007 0.035 0.079 0.172 0.364 0.799 1.518 2.021

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.