Rat Contactin-1 ELISA Kit PicoKine®

Contactin 1, also known as CNTN1, is a protein which in humans is encoded by the CNTN1 gene. It is mapped to 7q35. The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI) -anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 217 222 227 247 228 11.38 4.9% Sample 2 1103 1131 1250 1268 1188 71.96 6.0% Sample 3 3628 4052 4148 3796 3906 205.70 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 217 222 227 247 228 11.38 4.9% Sample 1 217 222 227 247 228 11.38 4.9% Sample 2 1103 1131 1250 1268 1188 71.96 6.0% Sample 2 1103 1131 1250 1268 1188 71.96 6.0% Sample 3 3628 4052 4148 3796 3906 205.70 5.2% Sample 3 3628 4052 4148 3796 3906 205.70 5.2%

Expressed by neurons, oligodendrocytes and their progenitors. Myelination regulates the expression being downregualted when neurons are in contact with Schwann cells.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 217 1103 3628 265 1145 3921 Standard deviation 9.77 62.87 166.89 18.82 79.01 250.94 CV (%) 4.5% 5.7% 4.6% 7.1% 6.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 217 1103 3628 265 1145 3921 Standard deviation 9.77 62.87 166.89 18.82 79.01 250.94 CV (%) 4.5% 5.7% 4.6% 7.1% 6.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 217 1103 3628 265 1145 3921 Mean (pg/ml) 217 1103 3628 265 1145 3921 Standard deviation 9.77 62.87 166.89 18.82 79.01 250.94 Standard deviation 9.77 62.87 166.89 18.82 79.01 250.94 CV (%) 4.5% 5.7% 4.6% 7.1% 6.9% 6.4% CV (%) 4.5% 5.7% 4.6% 7.1% 6.9% 6.4%

Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.003 0.027 0.062 0.125 0.279 0.553 1.201 2.148 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.003 0.027 0.062 0.125 0.279 0.553 1.201 2.148 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.003 0.027 0.062 0.125 0.279 0.553 1.201 2.148 O.D. 0.003 0.027 0.062 0.125 0.279 0.553 1.201 2.148

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.