Human IL-1RAcP/IL-1R3/IL1RAP ELISA Kit PicoKine®

Interleukin-1 receptor accessory protein is a protein that in humans is encoded by the IL1RAP gene. It is mapped to 3q28. This gene encodes a component of the interleukin 1 receptor complex, which initiates signalling events that result in the activation of interleukin 1-responsive genes. Alternative splicing of this gene results in membrane-bound and soluble isoforms differing in their C-terminus. The ratio of soluble to membrane-bound forms increases during acute-phase induction or stress.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 402 449 413 416 420 17.53 4.1% Sample 2 1364 1472 1581 1582 1499 90.22 6.0% Sample 3 5883 5867 6024 6394 6042 212.21 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 402 449 413 416 420 17.53 4.1% Sample 1 402 449 413 416 420 17.53 4.1% Sample 2 1364 1472 1581 1582 1499 90.22 6.0% Sample 2 1364 1472 1581 1582 1499 90.22 6.0% Sample 3 5883 5867 6024 6394 6042 212.21 3.5% Sample 3 5883 5867 6024 6394 6042 212.21 3.5%

Detected in liver, skin, placenta, thymus and lung. Isoform 4 is predominantly expressed in brain. Overexpressed on candidate chronic myeloid leukemia (CML) stem cells, hematopoietic stem cells and mononuclear cells of patients with acute myeloid leukemia (AML) . Overexpressed in patients with chronic obstructive pulmonary disease (COPD) . Expressed in T-helper 1 (Th1) and T-helper 2 (Th2) cell subsets.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 402 1364 5883 413 1472 6042 Standard deviation 18.49 77.75 270.62 21.06 86.85 386.69 CV (%) 4.6% 5.7% 4.6% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 402 1364 5883 413 1472 6042 Standard deviation 18.49 77.75 270.62 21.06 86.85 386.69 CV (%) 4.6% 5.7% 4.6% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 402 1364 5883 413 1472 6042 Mean (pg/ml) 402 1364 5883 413 1472 6042 Standard deviation 18.49 77.75 270.62 21.06 86.85 386.69 Standard deviation 18.49 77.75 270.62 21.06 86.85 386.69 CV (%) 4.6% 5.7% 4.6% 5.1% 5.9% 6.4% CV (%) 4.6% 5.7% 4.6% 5.1% 5.9% 6.4%

Coreceptor for IL1RL2 in the IL-36 signaling system. Coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Coreceptor for IL1RL1 in the IL-33 signaling system. Can biionally induce pre- and postsynaptic differentiation of neurons by trans-synaptically binding to PTPRD. May play a role in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells (Probable) .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.006 0.072 0.119 0.211 0.432 0.793 1.364 2.291 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.006 0.072 0.119 0.211 0.432 0.793 1.364 2.291 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.006 0.072 0.119 0.211 0.432 0.793 1.364 2.291 O.D. 0.006 0.072 0.119 0.211 0.432 0.793 1.364 2.291

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.