Mouse CXCL17 ELISA Kit PicoKine®

Chemokine (C-X-C motif) ligand 17 (CXCL17) is a small cytokine belonging to the CXC chemokine family that has been identified in humans and mice. It is mapped to 7; 7 A3. The protein encoded by this gene is a mucosal chemokine that attracts immature dendritic cells and blood monocytes to the lungs. The encoded protein also promotes tumorigenesis through an angiogenic activity. Finally, this protein exhibits strong antimicrobial activity against E. coli, S. aureus, Salmonella, P. aeruginosa, and C. albicans. Two transcript variants, one protein-coding and the other non-protein coding, have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 254 163 309 180 227 7.59 3.4% Sample 2 313 396 389 313 353 24.27 6.9% Sample 3 3010 4228 2934 3528 3425 160.29 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 254 163 309 180 227 7.59 3.4% Sample 1 254 163 309 180 227 7.59 3.4% Sample 2 313 396 389 313 353 24.27 6.9% Sample 2 313 396 389 313 353 24.27 6.9% Sample 3 3010 4228 2934 3528 3425 160.29 4.7% Sample 3 3010 4228 2934 3528 3425 160.29 4.7%

Detected in lung, trachea, lung, tongue thyroid, submaxillary gland, epididymis, and uterus tissues and at a lower level in ovary, prostate and in intestinal tissues.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 281 647 4090 210 852 4469 Standard deviation 22.14 18.12 305.93 10.58 23.6 156.86 CV (%) 7.9% 2.8% 7.5% 5.0% 2.8% 3.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 281 647 4090 210 852 4469 Standard deviation 22.14 18.12 305.93 10.58 23.6 156.86 CV (%) 7.9% 2.8% 7.5% 5.0% 2.8% 3.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 281 647 4090 210 852 4469 Mean (pg/ml) 281 647 4090 210 852 4469 Standard deviation 22.14 18.12 305.93 10.58 23.6 156.86 Standard deviation 22.14 18.12 305.93 10.58 23.6 156.86 CV (%) 7.9% 2.8% 7.5% 5.0% 2.8% 3.5% CV (%) 7.9% 2.8% 7.5% 5.0% 2.8% 3.5%

Chemokine that acts as chemoattractant for monocytes, macrophages and dendritic cells (PubMed:24973458) . Plays a role in angiogenesis and possibly in the development of tumors (PubMed:16989774) . Acts as an anti-inflammatory in the stomach. May play a role in the innate defense against infections. Activates the C-X-C chemokine receptor GPR35 to induce a rapid and transient rise in the level of intracellular calcium ions.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.041 0.122 0.193 0.341 0.598 0.952 1.531 2.256 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.041 0.122 0.193 0.341 0.598 0.952 1.531 2.256 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.041 0.122 0.193 0.341 0.598 0.952 1.531 2.256 O.D. 0.041 0.122 0.193 0.341 0.598 0.952 1.531 2.256

Aliquot 0.1 ml per well of the 10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml mouse CXCL17 standard solutions into the precoated 96-well plate. Add 0.1 ml of the sample diluent buffer into the control well (Zero well) . Add 0.1 ml of each properly diluted sample of mouse cell culture supernatants, serum or plasma (heparin, EDTA, citrate) to each empty well. See “Sample Dilution Guideline” above for details. We recommended that each mouse CXCL17 standard solution and each sample be measured in duplicate.

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.