Human TINAGL1 ELISA Kit PicoKine®

Tubulointerstitial nephritis antigen-like is a protein that in humans is encoded by the TINAGL1 gene. It is mapped to 1p35.2. The protein encoded by this gene is similar in sequence to tubulointerstitial nephritis antigen, a secreted glycoprotein that is recognized by antibodies in some types of immune-related tubulointerstitial nephritis. Three transcript variants encoding different isoforms have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 43 43 44 50 45 2.91 6.4% Sample 2 216 209 214 213 213 2.54 1.1% Sample 3 672 709 636 661 669 26.27 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 43 43 44 50 45 2.91 6.4% Sample 1 43 43 44 50 45 2.91 6.4% Sample 2 216 209 214 213 213 2.54 1.1% Sample 2 216 209 214 213 213 2.54 1.1% Sample 3 672 709 636 661 669 26.27 3.9% Sample 3 672 709 636 661 669 26.27 3.9%

Highly expressed in aorta, heart, placenta, kidney and a colorectal adenocarcinoma cell line. Moderately expressed in skeletal muscle, pancreas, lung, lymph nodes, adrenal gland, bone marrow and thyroid. Weakly expressed in colon, small intestine, ovary, spleen, testis and prostate. Predominantly found in vascular smooth muscle cells, but also in cardiac and skeletal muscle cells as well as kidney.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 43 216 672 52 225 857 Standard deviation 1.76 12.31 30.91 3.02 15.53 54.85 CV (%) 4.1% 5.7% 4.6% 5.8% 6.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 43 216 672 52 225 857 Standard deviation 1.76 12.31 30.91 3.02 15.53 54.85 CV (%) 4.1% 5.7% 4.6% 5.8% 6.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 43 216 672 52 225 857 Mean (pg/ml) 43 216 672 52 225 857 Standard deviation 1.76 12.31 30.91 3.02 15.53 54.85 Standard deviation 1.76 12.31 30.91 3.02 15.53 54.85 CV (%) 4.1% 5.7% 4.6% 5.8% 6.9% 6.4% CV (%) 4.1% 5.7% 4.6% 5.8% 6.9% 6.4%

May be implicated in the adrenocortical zonation and in mechanisms for repressing the CYP11B1 gene expression in adrenocortical cells. This is a non catalytic peptidase C1 family protein.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.058 0.128 0.186 0.276 0.467 0.735 1.359 2.243 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.058 0.128 0.186 0.276 0.467 0.735 1.359 2.243 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.058 0.128 0.186 0.276 0.467 0.735 1.359 2.243 O.D. 0.058 0.128 0.186 0.276 0.467 0.735 1.359 2.243

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.