Rat Serpin A12 ELISA Kit PicoKine®

Rat Serpin A12 ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Serpina12 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 19 17 16 17 1.11 6.5% Sample 2 79 82 78 92 82 5.53 6.7% Sample 3 426 461 468 504 464 27.68 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 19 17 16 17 1.11 6.5% Sample 1 18 19 17 16 17 1.11 6.5% Sample 2 79 82 78 92 82 5.53 6.7% Sample 2 79 82 78 92 82 5.53 6.7% Sample 3 426 461 468 504 464 27.68 5.9% Sample 3 426 461 468 504 464 27.68 5.9%

Synonyms

Serpin A12; Visceral adipose tissue-derived serine protease inhibitor; Vaspin; Visceral adipose-specific serpin; Serpina12

Gene Name

Serpina12

Gene ID

191570

UniProt

Q8R4Z1

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L21-P411

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Expressed in visceral adipose tissues.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 79 426 25 86 439 Standard deviation 0.86 4.50 19.60 1.28 5.07 28.10 CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 79 426 25 86 439 Standard deviation 0.86 4.50 19.60 1.28 5.07 28.10 CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 18 79 426 25 86 439 Mean (pg/ml) 18 79 426 25 86 439 Standard deviation 0.86 4.50 19.60 1.28 5.07 28.10 Standard deviation 0.86 4.50 19.60 1.28 5.07 28.10 CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4% CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4%

Sensitivity

<10 pg/mL

Function

Adipokine that modulates insulin action by specifically inhibiting its target protease KLK7 in white adipose tissues.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant rat Serpin A12

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Serpin family A member 12

Subcellular Location

Secreted.

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.041 0.126 0.194 0.331 0.605 1.025 1.698 2.34 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.041 0.126 0.194 0.331 0.605 1.025 1.698 2.34 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.041 0.126 0.194 0.331 0.605 1.025 1.698 2.34 O.D. 0.041 0.126 0.194 0.331 0.605 1.025 1.698 2.34

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.