Human IL-17RD ELISA Kit PicoKine®

Interleukin 17 receptor D is a protein that in humans is encoded by the IL17RD gene. It is mapped to 3p14.3. This gene encodes a membrane protein belonging to the interleukin-17 receptor (IL-17R) protein family. The encoded protein is a component of the interleukin-17 receptor signaling complex, and the interaction between this protein and IL-17R does not require the interleukin. The gene product also affects fibroblast growth factor signaling, inhibiting or stimulating growth through MAPK/ERK signaling. Alternate splicing generates multiple transcript variants encoding distinct isoforms.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 177 204 173 201 188 13.86 7.3% Sample 2 1044 1242 1097 1187 1142 76.89 6.7% Sample 3 3336 3119 3404 2806 3166 233.10 7.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 177 204 173 201 188 13.86 7.3% Sample 1 177 204 173 201 188 13.86 7.3% Sample 2 1044 1242 1097 1187 1142 76.89 6.7% Sample 2 1044 1242 1097 1187 1142 76.89 6.7% Sample 3 3336 3119 3404 2806 3166 233.10 7.3% Sample 3 3336 3119 3404 2806 3166 233.10 7.3%

Interleukin-17 receptor D; IL-17 receptor D; IL-17RD; IL17Rhom; Interleukin-17 receptor-like protein; Sef homolog; hSef; IL17RD; IL17RLM; SEF; UNQ6115; PRO20026

Expressed in umbilical vein endothelial cells and in several highly vascularized tissues such as kidney, colon, skeletal muscle, heart and small intestine. Highly expressed in ductal epithelial cells of salivary glands, seminal vesicles and the collecting tubules of the kidney. Isoform 1 is also highly expressed in both fetal and adult brain, pituitary, tonsils, spleen, adenoids, fetal kidney, liver, testes and ovary. Isoform 1 is also expressed at moderate levels in primary aortic endothelial cells and adrenal medulla, and at low levels in adrenal cortex. Isoform 4 is specifically and highly expressed in pituitary, fetal brain and umbilical vein endothelial cells.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 177 1044 3336 195 1133 4471 Standard deviation 9.03 55.33 183.48 11.31 70.25 295.09 CV (%) 5.1% 5.3% 5.5% 5.8% 6.2% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 177 1044 3336 195 1133 4471 Standard deviation 9.03 55.33 183.48 11.31 70.25 295.09 CV (%) 5.1% 5.3% 5.5% 5.8% 6.2% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 177 1044 3336 195 1133 4471 Mean (pg/ml) 177 1044 3336 195 1133 4471 Standard deviation 9.03 55.33 183.48 11.31 70.25 295.09 Standard deviation 9.03 55.33 183.48 11.31 70.25 295.09 CV (%) 5.1% 5.3% 5.5% 5.8% 6.2% 6.6% CV (%) 5.1% 5.3% 5.5% 5.8% 6.2% 6.6%

Feedback inhibitor of fibroblast growth factor mediated Ras-MAPK signaling and ERK activation. May inhibit FGF-induced FGFR1 tyrosine phosphorylation. Regulates the nuclear ERK signaling pathway by spatially blocking nuclear translocation of activated ERK without inhibiting cytoplasmic phosphorylation of ERK. Mediates JNK activation and may be involved in apoptosis. Might have a role in the early stages of fate specification of GnRH-secreting neurons.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.062 0.144 0.218 0.378 0.612 0.939 1.446 2.059 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.062 0.144 0.218 0.378 0.612 0.939 1.446 2.059 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.062 0.144 0.218 0.378 0.612 0.939 1.446 2.059 O.D. 0.062 0.144 0.218 0.378 0.612 0.939 1.446 2.059

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.