Rat DNER ELISA Kit PicoKine®

Rat DNER ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Dner in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Delta and Notch-like epidermal growth factor-related receptor is a protein that in humans is encoded by the DNER gene. It is mapped to 9q35. DNER, also known as BET, is a type I transmembrane glycoprotein that is specifically expressed on nonaxonal areas of post-mitotic neurons. The protein has an extracellular domain containing ten distinct EGF-like repeats similar to those found on Delta and Notch. Human and mouse DNER share 90% amino acid sequence identity.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 374 333 332 377 354 21.52 6.0% Sample 2 1696 1582 1441 1656 1593 97.21 6.1% Sample 3 6130 6281 6503 6976 6472 319.53 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 374 333 332 377 354 21.52 6.0% Sample 1 374 333 332 377 354 21.52 6.0% Sample 2 1696 1582 1441 1656 1593 97.21 6.1% Sample 2 1696 1582 1441 1656 1593 97.21 6.1% Sample 3 6130 6281 6503 6976 6472 319.53 4.9% Sample 3 6130 6281 6503 6976 6472 319.53 4.9%

Synonyms

Delta/notch-like EGF repeat containing; Dner

Gene Name

Dner

Gene ID

316573

UniProt

Q5U215

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A26-Y649

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 374 1696 6130 451 1824 8468 Standard deviation 17.95 79.71 300.37 23.90 98.50 567.36 CV (%) 4.8% 4.7% 4.9% 5.3% 5.4% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 374 1696 6130 451 1824 8468 Standard deviation 17.95 79.71 300.37 23.90 98.50 567.36 CV (%) 4.8% 4.7% 4.9% 5.3% 5.4% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 374 1696 6130 451 1824 8468 Mean (pg/ml) 374 1696 6130 451 1824 8468 Standard deviation 17.95 79.71 300.37 23.90 98.50 567.36 Standard deviation 17.95 79.71 300.37 23.90 98.50 567.36 CV (%) 4.8% 4.7% 4.9% 5.3% 5.4% 6.7% CV (%) 4.8% 4.7% 4.9% 5.3% 5.4% 6.7%

Sensitivity

<50 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant rat DNER

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Delta/notch-like EGF repeat containing

Subcellular Location

Integral component of membrane.

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.052 0.18 0.282 0.493 0.827 1.232 1.729 2.407 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.052 0.18 0.282 0.493 0.827 1.232 1.729 2.407 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.052 0.18 0.282 0.493 0.827 1.232 1.729 2.407 O.D. 0.052 0.18 0.282 0.493 0.827 1.232 1.729 2.407

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.