Mouse DNER ELISA Kit PicoKine®

Delta and Notch-like epidermal growth factor-related receptor is a protein that in humans is encoded by the DNER gene. It is mapped to 1; 1 C5. DNER, also known as BET, is a type I transmembrane glycoprotein that is specifically expressed on nonaxonal areas of post-mitotic neurons. The protein has an extracellular domain containing ten distinct EGF-like repeats similar to those found on Delta and Notch. Human and mouse DNER share 90% amino acid sequence identity.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 362 358 322 337 344 16.20 4.7% Sample 2 1554 1714 1606 1723 1649 71.71 4.3% Sample 3 8817 9078 8422 9894 9052 538.92 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 362 358 322 337 344 16.20 4.7% Sample 1 362 358 322 337 344 16.20 4.7% Sample 2 1554 1714 1606 1723 1649 71.71 4.3% Sample 2 1554 1714 1606 1723 1649 71.71 4.3% Sample 3 8817 9078 8422 9894 9052 538.92 5.9% Sample 3 8817 9078 8422 9894 9052 538.92 5.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 362 1554 8817 431 1816 9475 Standard deviation 17.74 82.36 476.12 28.45 123.49 748.53 CV (%) 4.9% 5.3% 5.4% 6.6% 6.8% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 362 1554 8817 431 1816 9475 Standard deviation 17.74 82.36 476.12 28.45 123.49 748.53 CV (%) 4.9% 5.3% 5.4% 6.6% 6.8% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 362 1554 8817 431 1816 9475 Mean (pg/ml) 362 1554 8817 431 1816 9475 Standard deviation 17.74 82.36 476.12 28.45 123.49 748.53 Standard deviation 17.74 82.36 476.12 28.45 123.49 748.53 CV (%) 4.9% 5.3% 5.4% 6.6% 6.8% 7.9% CV (%) 4.9% 5.3% 5.4% 6.6% 6.8% 7.9%

Mediates neuron-glia interaction during astrocytogenesis. May promote differentiation of Bergmann glia during cerebellar development by activating DELTEX-dependent NOTCH1 signaling.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.006 0.037 0.071 0.14 0.253 0.483 0.922 1.81 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.006 0.037 0.071 0.14 0.253 0.483 0.922 1.81 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.006 0.037 0.071 0.14 0.253 0.483 0.922 1.81 O.D. 0.006 0.037 0.071 0.14 0.253 0.483 0.922 1.81

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.