Human FGFRL1 ELISA Kit PicoKine®

Fibroblast growth factor receptor-like 1 is a protein that in humans is encoded by the FGFRL1 gene. It is mapped to 4p16.3. The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. A marked difference between this gene product and the other family members is its lack of a cytoplasmic tyrosine kinase domain. The result is a transmembrane receptor that could interact with other family members and potentially inhibit signaling. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 19 21 20 19 19 0.82 4.3% Sample 2 106 116 116 104 110 5.54 5.0% Sample 3 348 310 337 365 340 19.98 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 19 21 20 19 19 0.82 4.3% Sample 1 19 21 20 19 19 0.82 4.3% Sample 2 106 116 116 104 110 5.54 5.0% Sample 2 106 116 116 104 110 5.54 5.0% Sample 3 348 310 337 365 340 19.98 5.8% Sample 3 348 310 337 365 340 19.98 5.8%

Fibroblast growth factor receptor-like 1; FGF receptor-like protein 1; FGF homologous factor receptor; FGFR-like protein; Fibroblast growth factor receptor 5; FGFR-5; FGFRL1; FGFR5, FHFR; UNQ480; PRO943

Expressed preferentially in cartilaginous tissues and pancreas. Highly expressed in the liver, kidney, heart, brain and skeletal muscle. Weakly expressed in the lung, small intestine and spleen.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 19 106 348 23 118 418 Standard deviation 0.78 4.98 17.05 1.17 6.49 26.33 CV (%) 4.1% 4.7% 4.9% 5.1% 5.5% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 19 106 348 23 118 418 Standard deviation 0.78 4.98 17.05 1.17 6.49 26.33 CV (%) 4.1% 4.7% 4.9% 5.1% 5.5% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 19 106 348 23 118 418 Mean (pg/ml) 19 106 348 23 118 418 Standard deviation 0.78 4.98 17.05 1.17 6.49 26.33 Standard deviation 0.78 4.98 17.05 1.17 6.49 26.33 CV (%) 4.1% 4.7% 4.9% 5.1% 5.5% 6.3% CV (%) 4.1% 4.7% 4.9% 5.1% 5.5% 6.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.052 0.103 0.145 0.231 0.396 0.719 1.197 1.964 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.052 0.103 0.145 0.231 0.396 0.719 1.197 1.964 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.052 0.103 0.145 0.231 0.396 0.719 1.197 1.964 O.D. 0.052 0.103 0.145 0.231 0.396 0.719 1.197 1.964

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.