Human ARSB/Arylsulfatase B ELISA Kit PicoKine®

Arylsulfatase B is an enzyme associated with mucopolysaccharidosis VI. It is mapped to 5q14.1. Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targeted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 142 155 146 133 144 7.9 5.4% Sample 2 555 583 584 618 585 22.32 3.8% Sample 3 1654 1616 1741 1741 1688 54.67 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 142 155 146 133 144 7.9 5.4% Sample 1 142 155 146 133 144 7.9 5.4% Sample 2 555 583 584 618 585 22.32 3.8% Sample 2 555 583 584 618 585 22.32 3.8% Sample 3 1654 1616 1741 1741 1688 54.67 3.2% Sample 3 1654 1616 1741 1741 1688 54.67 3.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 142 555 1654 131 526 1652 Standard deviation 8.94 25.53 77.73 8.25 29.98 90.86 CV (%) 6.3% 4.6% 4.7% 6.3% 5.7% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 142 555 1654 131 526 1652 Standard deviation 8.94 25.53 77.73 8.25 29.98 90.86 CV (%) 6.3% 4.6% 4.7% 6.3% 5.7% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 142 555 1654 131 526 1652 Mean (pg/ml) 142 555 1654 131 526 1652 Standard deviation 8.94 25.53 77.73 8.25 29.98 90.86 Standard deviation 8.94 25.53 77.73 8.25 29.98 90.86 CV (%) 6.3% 4.6% 4.7% 6.3% 5.7% 5.5% CV (%) 6.3% 4.6% 4.7% 6.3% 5.7% 5.5%

Removes sulfate groups from chondroitin-4-sulfate (C4S) and regulates its degradation. Involved in the regulation of cell adhesion, cell migration and invasion in colonic epithelium. In the central nervous system, is a regulator of neurite outgrowth and neuronal plasticity, acting through the control of sulfate glycosaminoglycans and neurocan levels.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.002 0.102 0.189 0.36 0.66 1.042 1.707 2.317 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.002 0.102 0.189 0.36 0.66 1.042 1.707 2.317 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.002 0.102 0.189 0.36 0.66 1.042 1.707 2.317 O.D. 0.002 0.102 0.189 0.36 0.66 1.042 1.707 2.317

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.