Human Arylsulfatase A/ARSA ELISA Kit PicoKine®

Human ARSA ELISA Kit PicoKine® (96 Tests) . Quantitate Human ARSA in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Arylsulfatase A (or cerebroside-sulfatase) is an enzyme that breaks down sulfatides, namely cerebroside 3-sulfate into cerebroside and sulfate. In humans, arylsulfatase A is encoded by the ARSA gene. It is mapped to 22q13.33. The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 575 631 592 627 606 23.57 3.8% Sample 2 1965 2125 2005 2238 2083 107.00 5.1% Sample 3 6227 6092 6463 5971 6188 182.65 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 575 631 592 627 606 23.57 3.8% Sample 1 575 631 592 627 606 23.57 3.8% Sample 2 1965 2125 2005 2238 2083 107.00 5.1% Sample 2 1965 2125 2005 2238 2083 107.00 5.1% Sample 3 6227 6092 6463 5971 6188 182.65 2.9% Sample 3 6227 6092 6463 5971 6188 182.65 2.9%

Synonyms

Arylsulfatase A; ASA; Cerebroside-sulfatase; ARSA

Gene Name

ARSA

Gene ID

410

UniProt

P15289

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: R19-A507

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 575 1965 6227 581 2018 7737 Standard deviation 25.30 90.39 292.67 32.54 117.04 487.43 CV (%) 4.4% 4.6% 4.7% 5.6% 5.8% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 575 1965 6227 581 2018 7737 Standard deviation 25.30 90.39 292.67 32.54 117.04 487.43 CV (%) 4.4% 4.6% 4.7% 5.6% 5.8% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 575 1965 6227 581 2018 7737 Mean (pg/ml) 575 1965 6227 581 2018 7737 Standard deviation 25.30 90.39 292.67 32.54 117.04 487.43 Standard deviation 25.30 90.39 292.67 32.54 117.04 487.43 CV (%) 4.4% 4.6% 4.7% 5.6% 5.8% 6.3% CV (%) 4.4% 4.6% 4.7% 5.6% 5.8% 6.3%

Sensitivity

<50 pg/mL

Function

Hydrolyzes cerebroside sulfate.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human ARSA

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Arylsulfatase A

Subcellular Location

Endoplasmic reticulum. Lysosome.

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.075 0.16 0.205 0.312 0.556 0.935 1.497 2.272 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.075 0.16 0.205 0.312 0.556 0.935 1.497 2.272 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.075 0.16 0.205 0.312 0.556 0.935 1.497 2.272 O.D. 0.075 0.16 0.205 0.312 0.556 0.935 1.497 2.272

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.