Human GFRA3 ELISA Kit PicoKine®

GDNF family receptor alpha-3 (GFRα3), also known as the artemin receptor, is a protein that in humans is encoded by the GFRA3 gene. It is mapped to 5q31.2. The protein encoded by this gene is a glycosylphosphatidylinositol (GPI) -linked cell surface receptor and a member of the GDNF receptor family. It forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 49 53 50 54 51 2.06 4.0% Sample 2 225 222 233 208 222 9.02 4.0% Sample 3 1375 1355 1340 1235 1326 54.12 4.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 49 53 50 54 51 2.06 4.0% Sample 1 49 53 50 54 51 2.06 4.0% Sample 2 225 222 233 208 222 9.02 4.0% Sample 2 225 222 233 208 222 9.02 4.0% Sample 3 1375 1355 1340 1235 1326 54.12 4.0% Sample 3 1375 1355 1340 1235 1326 54.12 4.0%

Widely expressed in adult and fetus which exhibit a similar pattern. Essentially not expressed in the central nervous system, but highly expressed in several sensory and sympathetic ganglia of the peripheral nervous system. Moderate expression in many non-neuronal tissues, particularly those of the digestive and urogenital systems, but high expression in stomach and appendix. Several types of glandular tissues show low expression. Very low or no expression detected in the hematopoietic system.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 49 225 1375 62 303 1421 Standard deviation 2.50 11.93 75.63 4.09 20.60 102.31 CV (%) 5.1% 5.3% 5.5% 6.6% 6.8% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 49 225 1375 62 303 1421 Standard deviation 2.50 11.93 75.63 4.09 20.60 102.31 CV (%) 5.1% 5.3% 5.5% 6.6% 6.8% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 49 225 1375 62 303 1421 Mean (pg/ml) 49 225 1375 62 303 1421 Standard deviation 2.50 11.93 75.63 4.09 20.60 102.31 Standard deviation 2.50 11.93 75.63 4.09 20.60 102.31 CV (%) 5.1% 5.3% 5.5% 6.6% 6.8% 7.2% CV (%) 5.1% 5.3% 5.5% 6.6% 6.8% 7.2%

Receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin) . Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.051 0.139 0.212 0.366 0.527 0.91 1.422 1.919 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.051 0.139 0.212 0.366 0.527 0.91 1.422 1.919 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.051 0.139 0.212 0.366 0.527 0.91 1.422 1.919 O.D. 0.051 0.139 0.212 0.366 0.527 0.91 1.422 1.919

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.