Rat GFRA2 ELISA Kit PicoKine®

GDNF family receptor alpha-2 (GFRα2), also known as the neurturin receptor, is a protein that in humans is encoded by the GFRA2 gene. It is mapped to 15p11. The GFRA2 protein is a glycosylphosphatidylinositol (GPI) -linked cell surface receptor. It is part of the GDNF receptor family. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. They both bind the GFRA2 receptor. The receptor mediates activation of the RET tyrosine kinase receptor. This encoded protein acts preferentially as a receptor for NTN compared to its other family member, GDNF family receptor alpha 1. This gene is a candidate gene for RET-associated diseases. GFRA2 (gene) has been shown to interact with GDNF.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 478 504 491 512 496 12.93 2.6% Sample 2 1856 1844 1896 2084 1920 96.62 5.0% Sample 3 5549 5354 6042 6100 5761 317.99 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 478 504 491 512 496 12.93 2.6% Sample 1 478 504 491 512 496 12.93 2.6% Sample 2 1856 1844 1896 2084 1920 96.62 5.0% Sample 2 1856 1844 1896 2084 1920 96.62 5.0% Sample 3 5549 5354 6042 6100 5761 317.99 5.5% Sample 3 5549 5354 6042 6100 5761 317.99 5.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 478 1856 5549 564 2008 6234 Standard deviation 22.94 96.51 299.65 34.40 126.50 411.44 CV (%) 4.8% 5.2% 5.4% 6.1% 6.3% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 478 1856 5549 564 2008 6234 Standard deviation 22.94 96.51 299.65 34.40 126.50 411.44 CV (%) 4.8% 5.2% 5.4% 6.1% 6.3% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 478 1856 5549 564 2008 6234 Mean (pg/ml) 478 1856 5549 564 2008 6234 Standard deviation 22.94 96.51 299.65 34.40 126.50 411.44 Standard deviation 22.94 96.51 299.65 34.40 126.50 411.44 CV (%) 4.8% 5.2% 5.4% 6.1% 6.3% 6.6% CV (%) 4.8% 5.2% 5.4% 6.1% 6.3% 6.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.028 0.111 0.184 0.328 0.625 1.072 1.754 2.417 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.028 0.111 0.184 0.328 0.625 1.072 1.754 2.417 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.028 0.111 0.184 0.328 0.625 1.072 1.754 2.417 O.D. 0.028 0.111 0.184 0.328 0.625 1.072 1.754 2.417

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.