Mouse GFRA2 ELISA Kit PicoKine®

GDNF family receptor alpha-2 (GFRα2), also known as the neurturin receptor, is a protein that in humans is encoded by the GFRA2 gene. It is mapped to 14; 14 D2. The GFRA2 protein is a glycosylphosphatidylinositol (GPI) -linked cell surface receptor. It is part of the GDNF receptor family. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. They both bind the GFRA2 receptor. The receptor mediates activation of the RET tyrosine kinase receptor. This encoded protein acts preferentially as a receptor for NTN compared to its other family member, GDNF family receptor alpha 1. This gene is a candidate gene for RET-associated diseases. GFRA2 (gene) has been shown to interact with GDNF.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 536 499 569 494 524 30.38 5.7% Sample 2 2108 2258 2305 2207 2219 73.11 3.2% Sample 3 8768 8608 9463 9664 9125 447.07 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 536 499 569 494 524 30.38 5.7% Sample 1 536 499 569 494 524 30.38 5.7% Sample 2 2108 2258 2305 2207 2219 73.11 3.2% Sample 2 2108 2258 2305 2207 2219 73.11 3.2% Sample 3 8768 8608 9463 9664 9125 447.07 4.8% Sample 3 8768 8608 9463 9664 9125 447.07 4.8%

GDNF family receptor alpha-2; GDNF receptor alpha-2; GDNFR-alpha-2; GFR-alpha-2; GDNF receptor beta; GDNFR-beta; Neurturin receptor alpha; NRTNR-alpha; NTNR-alpha; TGF-beta-related neurotrophic factor receptor 2; Gfra2; Gdnfrb; Trnr2

Neurons of the superior cervical and dorsal root ganglia, and adult brain and testis. Low level in the substantia nigra, spleen and adrenal gland . Isoform 1, isoform 2 and isoform 3 are all expressed in brain, liver, ileum, spleen, heart and kidney. In brain, isoform 1 is most abundant, isoform 2 slightly less and isoform 3 is lowest. No significant levels of isoform 1, isoform 2 or isoform 3 expression in testis.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 536 2108 8768 582 2224 9685 Standard deviation 23.05 94.86 412.10 32.59 128.99 600.47 CV (%) 4.3% 4.5% 4.7% 5.6% 5.8% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 536 2108 8768 582 2224 9685 Standard deviation 23.05 94.86 412.10 32.59 128.99 600.47 CV (%) 4.3% 4.5% 4.7% 5.6% 5.8% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 536 2108 8768 582 2224 9685 Mean (pg/ml) 536 2108 8768 582 2224 9685 Standard deviation 23.05 94.86 412.10 32.59 128.99 600.47 Standard deviation 23.05 94.86 412.10 32.59 128.99 600.47 CV (%) 4.3% 4.5% 4.7% 5.6% 5.8% 6.2% CV (%) 4.3% 4.5% 4.7% 5.6% 5.8% 6.2%

Receptor for neurturin. Mediates the NRTN-induced autophosphorylation and activation of the RET receptor. Also able to mediate GDNF signaling through the RET tyrosine kinase receptor. Participates in NRTN-induced 'Ser-727' phosphorylation of STAT3.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.005 0.061 0.127 0.237 0.429 0.83 1.324 1.942 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.005 0.061 0.127 0.237 0.429 0.83 1.324 1.942 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.005 0.061 0.127 0.237 0.429 0.83 1.324 1.942 O.D. 0.005 0.061 0.127 0.237 0.429 0.83 1.324 1.942

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.