Human GFRA2 ELISA Kit PicoKine®

GDNF family receptor alpha-2 (GFRα2), also known as the neurturin receptor, is a protein that in humans is encoded by the GFRA2 gene. It is mapped to 8p21.3. The GFRA2 protein is a glycosylphosphatidylinositol (GPI) -linked cell surface receptor. It is part of the GDNF receptor family. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. They both bind the GFRA2 receptor. The receptor mediates activation of the RET tyrosine kinase receptor. This encoded protein acts preferentially as a receptor for NTN compared to its other family member, GDNF family receptor alpha 1. This gene is a candidate gene for RET-associated diseases. GFRA2 (gene) has been shown to interact with GDNF.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 400 410 427 424 415 10.89 2.6% Sample 2 1999 2143 1981 2279 2100 120.67 5.7% Sample 3 8889 8412 9946 9621 9217 602.13 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 400 410 427 424 415 10.89 2.6% Sample 1 400 410 427 424 415 10.89 2.6% Sample 2 1999 2143 1981 2279 2100 120.67 5.7% Sample 2 1999 2143 1981 2279 2100 120.67 5.7% Sample 3 8889 8412 9946 9621 9217 602.13 6.5% Sample 3 8889 8412 9946 9621 9217 602.13 6.5%

GDNF family receptor alpha-2; GDNF receptor alpha-2; GDNFR-alpha-2; GFR-alpha-2; GDNF receptor beta; GDNFR-beta; Neurturin receptor alpha; NRTNR-alpha; NTNR-alpha; RET ligand 2; TGF-beta-related neurotrophic factor receptor 2; GFRA2; GDNFRB; RETL2; TRNR2

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 400 1999 8889 457 2104 9928 Standard deviation 19.20 97.95 444.45 23.31 124.14 635.39 CV (%) 4.8% 4.9% 5.0% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 400 1999 8889 457 2104 9928 Standard deviation 19.20 97.95 444.45 23.31 124.14 635.39 CV (%) 4.8% 4.9% 5.0% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 400 1999 8889 457 2104 9928 Mean (pg/ml) 400 1999 8889 457 2104 9928 Standard deviation 19.20 97.95 444.45 23.31 124.14 635.39 Standard deviation 19.20 97.95 444.45 23.31 124.14 635.39 CV (%) 4.8% 4.9% 5.0% 5.1% 5.9% 6.4% CV (%) 4.8% 4.9% 5.0% 5.1% 5.9% 6.4%

Receptor for neurturin. Mediates the NRTN-induced autophosphorylation and activation of the RET receptor. Also able to mediate GDNF signaling through the RET tyrosine kinase receptor. Participates in NRTN-induced 'Ser-727' phosphorylation of STAT3.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.018 0.042 0.077 0.125 0.236 0.625 1.295 1.969 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.018 0.042 0.077 0.125 0.236 0.625 1.295 1.969 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.018 0.042 0.077 0.125 0.236 0.625 1.295 1.969 O.D. 0.018 0.042 0.077 0.125 0.236 0.625 1.295 1.969

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.