Rat GFRA1 ELISA Kit PicoKine®

GDNF family receptor alpha-1 (GFRα1), also known as the GDNF receptor, is a protein that in humans is encoded by the GFRA1 gene. It is mapped to 1q55. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI) -linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.GDNF family receptor alpha 1 has been shown to interact with GDNF[8][9] and RET proto-oncogene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 22 21 21 23 21 0.82 3.9% Sample 2 99 94 88 91 93 4.06 4.3% Sample 3 314 347 308 295 316 19.17 6.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 22 21 21 23 21 0.82 3.9% Sample 1 22 21 21 23 21 0.82 3.9% Sample 2 99 94 88 91 93 4.06 4.3% Sample 2 99 94 88 91 93 4.06 4.3% Sample 3 314 347 308 295 316 19.17 6.0% Sample 3 314 347 308 295 316 19.17 6.0%

GDNF family receptor alpha-1; GDNF receptor alpha-1; GDNFR-alpha-1; GFR-alpha-1; RET ligand 1; TGF-beta-related neurotrophic factor receptor 1; Gfra1; Gdnfra; Retl1; Trnr1

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 78 249 857 81 254 955 Standard deviation 3.20 11.45 45.42 4.46 17.02 71.63 CV (%) 4.1% 4.6% 5.3% 5.5% 6.7% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 78 249 857 81 254 955 Standard deviation 3.20 11.45 45.42 4.46 17.02 71.63 CV (%) 4.1% 4.6% 5.3% 5.5% 6.7% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 78 249 857 81 254 955 Mean (pg/ml) 78 249 857 81 254 955 Standard deviation 3.20 11.45 45.42 4.46 17.02 71.63 Standard deviation 3.20 11.45 45.42 4.46 17.02 71.63 CV (%) 4.1% 4.6% 5.3% 5.5% 6.7% 7.5% CV (%) 4.1% 4.6% 5.3% 5.5% 6.7% 7.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.004 0.116 0.197 0.351 0.605 1.049 1.764 2.818 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.004 0.116 0.197 0.351 0.605 1.049 1.764 2.818 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.004 0.116 0.197 0.351 0.605 1.049 1.764 2.818 O.D. 0.004 0.116 0.197 0.351 0.605 1.049 1.764 2.818

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.