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Mouse GFRA1 ELISA Kit PicoKine®

Mouse GFRA1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Gfra1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

GDNF family receptor alpha-1 (GFRα1), also known as the GDNF receptor, is a protein that in humans is encoded by the GFRA1 gene. It is mapped to 19 D2; 19 54.18 cM. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI) -linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.GDNF family receptor alpha 1 has been shown to interact with GDNF and RET proto-oncogene.

Anticoagulant

Heparin, EDTA or citrate

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 17 16 16 16 16 0.43 2.6% Sample 2 89 96 104 99 97 5.43 5.5% Sample 3 450 497 503 495 486 21.13 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 17 16 16 16 16 0.43 2.6% Sample 1 17 16 16 16 16 0.43 2.6% Sample 2 89 96 104 99 97 5.43 5.5% Sample 2 89 96 104 99 97 5.43 5.5% Sample 3 450 497 503 495 486 21.13 4.3% Sample 3 450 497 503 495 486 21.13 4.3%

Synonyms

GDNF family receptor alpha-1; GDNF receptor alpha-1; GDNFR-alpha-1; GFR-alpha-1; TGF-beta-related neurotrophic factor receptor 1; Gfra1; Gdnfra; Trnr1

Gene Name

Gfra1

Gene ID

14585

UniProt

P97785

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D25-S430

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Expressed in heart, brain, lung, liver, kidney and testis.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 78 249 857 81 254 955 Standard deviation 3.20 11.45 45.42 4.46 17.02 71.63 CV (%) 4.1% 4.6% 5.3% 5.5% 6.7% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 78 249 857 81 254 955 Standard deviation 3.20 11.45 45.42 4.46 17.02 71.63 CV (%) 4.1% 4.6% 5.3% 5.5% 6.7% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 78 249 857 81 254 955 Mean (pg/ml) 78 249 857 81 254 955 Standard deviation 3.20 11.45 45.42 4.46 17.02 71.63 Standard deviation 3.20 11.45 45.42 4.46 17.02 71.63 CV (%) 4.1% 4.6% 5.3% 5.5% 6.7% 7.5% CV (%) 4.1% 4.6% 5.3% 5.5% 6.7% 7.5%

Sensitivity

<10 pg/mL

Function

Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Product Datasheet

https://www.bosterbio.com/datasheet?sku=EK2070

Product MSDS

https://www.bosterbio.com/msds?sku=EK2070

Specificity

Natural and recombinant mouse GFRA1

Gene Name Synonym

Glial cell line derived neurotrophic factor family receptor alpha 1

Subcellular Location

Cell membrane. GPI-anchor.

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.07 0.143 0.197 0.292 0.464 0.793 1.212 2.025 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.07 0.143 0.197 0.292 0.464 0.793 1.212 2.025 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.07 0.143 0.197 0.292 0.464 0.793 1.212 2.025 O.D. 0.07 0.143 0.197 0.292 0.464 0.793 1.212 2.025

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
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