Mouse BR-Cadherin-12 Cdh12 ELISA Kit PicoKine®

Cadherin-12 is a protein that in humans is encoded by the CDH12 gene. It is mapped to 15 A2; 15 8.25 cM. This gene encodes a member of the cadherin family of calcium-dependent glycoproteins that mediate cell adhesion and regulate many morphogenetic events during development. The encoded preproprotein is further processed to generate a mature protein. This gene is expressed in both the excitatory and inhibitory neurons in various areas of the adult central nervous system including the cerebellum, neocortex, hippocampus and in different subcortical nuclei. Multiple distinct genes of the cadherin family, including this gene, are found on chromosome 15.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 169 180 186 171 176 6.87 3.9% Sample 2 933 882 941 864 905 32.74 3.6% Sample 3 2976 3195 3034 3038 3060 81.29 2.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 169 180 186 171 176 6.87 3.9% Sample 1 169 180 186 171 176 6.87 3.9% Sample 2 933 882 941 864 905 32.74 3.6% Sample 2 933 882 941 864 905 32.74 3.6% Sample 3 2976 3195 3034 3038 3060 81.29 2.6% Sample 3 2976 3195 3034 3038 3060 81.29 2.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 169 933 2976 183 1016 4238 Standard deviation 7.10 40.12 122.02 12.08 73.15 322.09 CV (%) 4.2% 4.3% 4.1% 6.6% 7.2% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 169 933 2976 183 1016 4238 Standard deviation 7.10 40.12 122.02 12.08 73.15 322.09 CV (%) 4.2% 4.3% 4.1% 6.6% 7.2% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 169 933 2976 183 1016 4238 Mean (pg/ml) 169 933 2976 183 1016 4238 Standard deviation 7.10 40.12 122.02 12.08 73.15 322.09 Standard deviation 7.10 40.12 122.02 12.08 73.15 322.09 CV (%) 4.2% 4.3% 4.1% 6.6% 7.2% 7.6% CV (%) 4.2% 4.3% 4.1% 6.6% 7.2% 7.6%

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.084 0.129 0.171 0.233 0.491 0.812 1.22 1.94 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.084 0.129 0.171 0.233 0.491 0.812 1.22 1.94 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.084 0.129 0.171 0.233 0.491 0.812 1.22 1.94 O.D. 0.084 0.129 0.171 0.233 0.491 0.812 1.22 1.94

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.