Human DCR2/TNFRSF10D ELISA Kit PicoKine®

Decoy receptor 2 (DCR2), also known as TRAIL receptor 4 (TRAILR4) and tumor necrosis factor receptor superfamily member 10D (TNFRSF10D), is a human cell surface receptor of the TNF-receptor superfamily. It is mapped to 8p21.3. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains an extracellular TRAIL-binding domain, a transmembrane domain, and a truncated cytoplasmic death domain. This receptor does not induce apoptosis, and has been shown to play an inhibitory role in TRAIL-induced cell apoptosis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 26 25 29 29 27 1.78 6.5% Sample 2 111 103 112 100 106 5.12 4.8% Sample 3 334 308 342 290 318 20.70 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 26 25 29 29 27 1.78 6.5% Sample 1 26 25 29 29 27 1.78 6.5% Sample 2 111 103 112 100 106 5.12 4.8% Sample 2 111 103 112 100 106 5.12 4.8% Sample 3 334 308 342 290 318 20.70 6.5% Sample 3 334 308 342 290 318 20.70 6.5%

Tumor necrosis factor receptor superfamily member 10D; Decoy receptor 2; DcR2; TNF-related apoptosis-inducing ligand receptor 4; TRAIL receptor 4; TRAIL-R4; TRAIL receptor with a truncated death domain; CD264; TNFRSF10D; DCR2, TRAILR4, TRUNDD; UNQ251; PRO288

Widely expressed, in particular in fetal kidney, lung and liver, and in adult testis and liver. Also expressed in peripheral blood leukocytes, colon and small intestine, ovary, prostate, thymus, spleen, pancreas, kidney, lung, placenta and heart.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 26 111 334 29 120 428 Standard deviation 1.09 4.88 15.36 1.48 7.08 27.39 CV (%) 4.2% 4.4% 4.6% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 26 111 334 29 120 428 Standard deviation 1.09 4.88 15.36 1.48 7.08 27.39 CV (%) 4.2% 4.4% 4.6% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 26 111 334 29 120 428 Mean (pg/ml) 26 111 334 29 120 428 Standard deviation 1.09 4.88 15.36 1.48 7.08 27.39 Standard deviation 1.09 4.88 15.36 1.48 7.08 27.39 CV (%) 4.2% 4.4% 4.6% 5.1% 5.9% 6.4% CV (%) 4.2% 4.4% 4.6% 5.1% 5.9% 6.4%

Receptor for the cytotoxic ligand TRAIL. Contains a truncated death domain and hence is not capable of inducing apoptosis but protects against TRAIL-mediated apoptosis. Reports are contradictory with regards to its ability to induce the NF-kappa-B pathway.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.032 0.085 0.143 0.248 0.423 0.816 1.359 2.231 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.032 0.085 0.143 0.248 0.423 0.816 1.359 2.231 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.032 0.085 0.143 0.248 0.423 0.816 1.359 2.231 O.D. 0.032 0.085 0.143 0.248 0.423 0.816 1.359 2.231

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.