Mouse RTN4R ELISA Kit PicoKine®

Reticulon 4 receptor (RTN4R) also known as Nogo-66 Receptor (NgR) or Nogo receptor 1 is a protein which in humans is encoded by the RTN4R gene. It is mapped to 16; 16 A3. This gene encodes the receptor for reticulon 4, oligodendrocytemyelin glycoprotein and myelin-associated glycoprotein. This receptor mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult central nervous system.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 301 299 261 285 286 15.96 5.5% Sample 2 1170 1314 1144 1295 1230 74.62 6.0% Sample 3 4798 4625 4283 4662 4592 189.67 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 301 299 261 285 286 15.96 5.5% Sample 1 301 299 261 285 286 15.96 5.5% Sample 2 1170 1314 1144 1295 1230 74.62 6.0% Sample 2 1170 1314 1144 1295 1230 74.62 6.0% Sample 3 4798 4625 4283 4662 4592 189.67 4.1% Sample 3 4798 4625 4283 4662 4592 189.67 4.1%

Detected in embryonic hippocampus neurons. Detected in brain. Detected in neurons in the neocortex, in hippocampus, dorsal thalamus, cerebellum granule cell layer and the mitral cell layer in the olfactory bulb. Detected in brain, dorsal root ganglion and heart.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 301 1170 4798 307 1195 4812 Standard deviation 15.65 62.01 259.09 18.73 70.51 327.22 CV (%) 5.2% 5.3% 5.4% 6.1% 5.9% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 301 1170 4798 307 1195 4812 Standard deviation 15.65 62.01 259.09 18.73 70.51 327.22 CV (%) 5.2% 5.3% 5.4% 6.1% 5.9% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 301 1170 4798 307 1195 4812 Mean (pg/ml) 301 1170 4798 307 1195 4812 Standard deviation 15.65 62.01 259.09 18.73 70.51 327.22 Standard deviation 15.65 62.01 259.09 18.73 70.51 327.22 CV (%) 5.2% 5.3% 5.4% 6.1% 5.9% 6.8% CV (%) 5.2% 5.3% 5.4% 6.1% 5.9% 6.8%

Receptor for RTN4, OMG and MAG. Functions as receptor for the sialylated gangliosides GT1b and GM1. Besides, functions as receptor for chondroitin sulfate proteoglycans. Can also bind heparin. Intracellular signaling cascades are triggered via the coreceptor NGFR. Signaling mediates activation of Rho and downstream reorganization of the actin cytoskeleton. Mediates axonal growth inhibition. Mediates axonal growth inhibition and plays a role in regulating axon regeneration and neuronal plasticity in the adult central nervous system. Plays a role in postnatal brain development. Required for normal axon migration across the brain midline and normal formation of the corpus callosum. Protects motoneurons against apoptosis; protection against apoptosis is probably mediated via interaction with MAG. Acts in conjunction with RTN4 and LINGO1 in regulating neuronal precursor cell motility during cortical development. Like other family members, plays a role in restricting the number dendritic spines and the number of synapses that are formed during brain development.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.032 0.051 0.085 0.143 0.248 0.523 1.016 1.959 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.032 0.051 0.085 0.143 0.248 0.523 1.016 1.959 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.032 0.051 0.085 0.143 0.248 0.523 1.016 1.959 O.D. 0.032 0.051 0.085 0.143 0.248 0.523 1.016 1.959

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.