Human Neutrophil Elastase/ELA2/ELANE ELISA Kit PicoKine®

Human Neutrophil Elastase/ELA2/ELANE ELISA Kit PicoKine® (96 Tests) . Quantitate Human ELANE in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

In humans, neutrophil elastase is encoded by the ELANE gene, which resides on chromosome 19. It is mapped to 19p13.3. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN) . This gene is present in a gene cluster on chromosome 19.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1174 1071 1108 1106 1114 37.23 3.3% Sample 2 5107 5193 4515 4791 4901 268.69 5.4% Sample 3 21947 21460 19663 20115 20796 937.16 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1174 1071 1108 1106 1114 37.23 3.3% Sample 1 1174 1071 1108 1106 1114 37.23 3.3% Sample 2 5107 5193 4515 4791 4901 268.69 5.4% Sample 2 5107 5193 4515 4791 4901 268.69 5.4% Sample 3 21947 21460 19663 20115 20796 937.16 4.5% Sample 3 21947 21460 19663 20115 20796 937.16 4.5%

Synonyms

Neutrophil elastase; Bone marrow serine protease; Elastase-2; Human leukocyte elastase; HLE; Medullasin; PMN elastase; ELANE; ELA2

Gene Name

ELANE

Gene ID

1991

UniProt

P08246

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: I30-H267

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Bone marrow cells.

Applications

ELISA

Standard

50 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

0.78 ng/mL - 50 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1174 5107 21947 1365 6165 23984 Standard deviation 48.13 214.49 987.62 64.16 332.91 1367.09 CV (%) 4.1% 4.2% 4.5% 4.7% 5.4% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1174 5107 21947 1365 6165 23984 Standard deviation 48.13 214.49 987.62 64.16 332.91 1367.09 CV (%) 4.1% 4.2% 4.5% 4.7% 5.4% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1174 5107 21947 1365 6165 23984 Mean (pg/ml) 1174 5107 21947 1365 6165 23984 Standard deviation 48.13 214.49 987.62 64.16 332.91 1367.09 Standard deviation 48.13 214.49 987.62 64.16 332.91 1367.09 CV (%) 4.1% 4.2% 4.5% 4.7% 5.4% 5.7% CV (%) 4.1% 4.2% 4.5% 4.7% 5.4% 5.7%

Sensitivity

<50 pg/mL

Function

Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human ELANE

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Elastase, neutrophil expressed

Subcellular Location

Collagen-containing extracellular matrix. Extracellular exosome. Extracellular region. Extracellular space. Azurophil granule lumen.

Sample Data

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.057 0.091 0.124 0.241 0.418 0.87 1.736 2.447 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.057 0.091 0.124 0.241 0.418 0.87 1.736 2.447 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.057 0.091 0.124 0.241 0.418 0.87 1.736 2.447 O.D. 0.057 0.091 0.124 0.241 0.418 0.87 1.736 2.447

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.