Mouse Vitamin D BP/GC/Vitamin D Binding Protein ELISA Kit PicoKine®

Vitamin D-binding protein (DBP), also/originally known as gc-globulin (group-specific component), is a protein that in humans is encoded by the GC gene. It is mapped to 5 E1; 5 44.32 cM. The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 551 505 496 595 536 39.57 7.3% Sample 2 2528 2531 2745 2357 2540 137.6 5.4% Sample 3 8207 9826 9283 9589 9226 619.14 6.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 551 505 496 595 536 39.57 7.3% Sample 1 551 505 496 595 536 39.57 7.3% Sample 2 2528 2531 2745 2357 2540 137.6 5.4% Sample 2 2528 2531 2745 2357 2540 137.6 5.4% Sample 3 8207 9826 9283 9589 9226 619.14 6.7% Sample 3 8207 9826 9283 9589 9226 619.14 6.7%

Vitamin D-binding protein; DBP; VDB; Gc protein-derived macrophage activating factor; Gc-MAF; GcMAF; Gc-globulin; Group-specific component; Gc; Vitamin D-binding protein-macrophage activating factor; DBP-maf; GC

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 551 2528 8207 568 2328 8686 Standard deviation 36.91 156.73 648.35 47.14 144.33 773.05 CV (%) 6.7% 6.2% 7.9% 8.3% 6.2% 8.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 551 2528 8207 568 2328 8686 Standard deviation 36.91 156.73 648.35 47.14 144.33 773.05 CV (%) 6.7% 6.2% 7.9% 8.3% 6.2% 8.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 551 2528 8207 568 2328 8686 Mean (pg/ml) 551 2528 8207 568 2328 8686 Standard deviation 36.91 156.73 648.35 47.14 144.33 773.05 Standard deviation 36.91 156.73 648.35 47.14 144.33 773.05 CV (%) 6.7% 6.2% 7.9% 8.3% 6.2% 8.9% CV (%) 6.7% 6.2% 7.9% 8.3% 6.2% 8.9%

Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.028 0.069 0.115 0.215 0.381 0.729 1.433 2.243 Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.028 0.069 0.115 0.215 0.381 0.729 1.433 2.243 Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.028 0.069 0.115 0.215 0.381 0.729 1.433 2.243 O.D. 0.028 0.069 0.115 0.215 0.381 0.729 1.433 2.243

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.