Human SEMA6D ELISA Kit PicoKine®

Semaphorin 6D (Sema6D) is a 130 ? 135 kDa member of the Semaphorin family of axon guidance molecules. This gene is mapped to 15q21.1. Semaphorins are a large family, including both secreted and membrane associated proteins, many of which have been implicated as inhibitors or chemorepellents in axon pathfinding, fasciculation and branching, and target selection. All semaphorins possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Additional sequence motifs C-terminal to the semaphorin domain allow classification into distinct subfamilies. Results demonstrate that transmembrane semaphorins, like the secreted ones, can act as repulsive axon guidance cues. This gene encodes a class 6 vertebrate transmembrane semaphorin that demonstrates alternative splicing. Several transcript variants have been identified and expression of the distinct encoded isoforms is thought to be regulated in a tissue- and development-dependent manner.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 51 51 47 53 50 2.17 4.3% Sample 2 231 236 248 275 247 17.03 6.8% Sample 3 958 1018 899 979 963 43.01 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 51 51 47 53 50 2.17 4.3% Sample 1 51 51 47 53 50 2.17 4.3% Sample 2 231 236 248 275 247 17.03 6.8% Sample 2 231 236 248 275 247 17.03 6.8% Sample 3 958 1018 899 979 963 43.01 4.4% Sample 3 958 1018 899 979 963 43.01 4.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 51 231 958 56 240 967 Standard deviation 3.01 9.93 39.28 3.70 13.92 73.49 CV (%) 5.9% 4.3% 4.1% 6.6% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 51 231 958 56 240 967 Standard deviation 3.01 9.93 39.28 3.70 13.92 73.49 CV (%) 5.9% 4.3% 4.1% 6.6% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 51 231 958 56 240 967 Mean (pg/ml) 51 231 958 56 240 967 Standard deviation 3.01 9.93 39.28 3.70 13.92 73.49 Standard deviation 3.01 9.93 39.28 3.70 13.92 73.49 CV (%) 5.9% 4.3% 4.1% 6.6% 5.8% 7.6% CV (%) 5.9% 4.3% 4.1% 6.6% 5.8% 7.6%

Shows growth cone collapsing activity on dorsal root ganglion (DRG) neurons in vitro. May be a stop signal for the DRG neurons in their target areas, and possibly also for other neurons. May also be involved in the maintenance and remodeling of neuronal connections.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.027 0.09 0.151 0.272 0.477 0.899 1.591 2.873 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.027 0.09 0.151 0.272 0.477 0.899 1.591 2.873 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.027 0.09 0.151 0.272 0.477 0.899 1.591 2.873 O.D. 0.027 0.09 0.151 0.272 0.477 0.899 1.591 2.873

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.