Rat SEMA6C ELISA Kit PicoKine®

Semaphorin-6C is a protein that in humans is encoded by the SEMA6C gene. This gene is mapped to 2q34. This gene product is a member of the semaphoring family of proteins. Semaphorins represent important molecular signals controlling multiple aspects of the cellular response that follows CNS injury, and thus may play an important role in neural regeneration.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 116 110 102 113 110 5.21 4.7% Sample 2 431 426 431 378 416 22.32 5.3% Sample 3 1934 1731 1763 1838 1816 78.16 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 116 110 102 113 110 5.21 4.7% Sample 1 116 110 102 113 110 5.21 4.7% Sample 2 431 426 431 378 416 22.32 5.3% Sample 2 431 426 431 378 416 22.32 5.3% Sample 3 1934 1731 1763 1838 1816 78.16 4.3% Sample 3 1934 1731 1763 1838 1816 78.16 4.3%

Expressed in many regions of the developing nervous system, probably in neurons and their precursors, but also in nonneural tissue such as immature muscle and dermis. In adult, strong expression in the skeletal muscle and moderate expression in the brain, where cerebellum shows the highest expression. Also expressed in almost all areas of the CNS.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 116 431 1934 121 478 1957 Standard deviation 6.73 25.86 88.96 7.38 32.98 142.86 CV (%) 5.8% 6.0% 4.6% 6.1% 6.9% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 116 431 1934 121 478 1957 Standard deviation 6.73 25.86 88.96 7.38 32.98 142.86 CV (%) 5.8% 6.0% 4.6% 6.1% 6.9% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 116 431 1934 121 478 1957 Mean (pg/ml) 116 431 1934 121 478 1957 Standard deviation 6.73 25.86 88.96 7.38 32.98 142.86 Standard deviation 6.73 25.86 88.96 7.38 32.98 142.86 CV (%) 5.8% 6.0% 4.6% 6.1% 6.9% 7.3% CV (%) 5.8% 6.0% 4.6% 6.1% 6.9% 7.3%

Shows growth cone collapsing activity on dorsal root ganglion (DRG) neurons in vitro. May be a stop signal for the DRG neurons in their target areas, and possibly also for other neurons. May also be involved in the maintenance and remodeling of neuronal connections.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.01 0.078 0.136 0.246 0.436 0.819 1.433 2.107 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.01 0.078 0.136 0.246 0.436 0.819 1.433 2.107 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.01 0.078 0.136 0.246 0.436 0.819 1.433 2.107 O.D. 0.01 0.078 0.136 0.246 0.436 0.819 1.433 2.107

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.