Mouse SEMA6C ELISA Kit PicoKine®

Mouse SEMA6C ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Sema6c in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Semaphorin-6C is a protein that in humans is encoded by the SEMA6C gene. This gene is mapped to 3; 3 F2.1. This gene product is a member of the semaphoring family of proteins. Semaphorins represent important molecular signals controlling multiple aspects of the cellular response that follows CNS injury, and thus may play an important role in neural regeneration.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 95 85 100 85 91 6.49 7.1% Sample 2 439 446 458 491 458 19.95 4.3% Sample 3 1073 1102 1194 1246 1153 69.51 6.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 95 85 100 85 91 6.49 7.1% Sample 1 95 85 100 85 91 6.49 7.1% Sample 2 439 446 458 491 458 19.95 4.3% Sample 2 439 446 458 491 458 19.95 4.3% Sample 3 1073 1102 1194 1246 1153 69.51 6.0% Sample 3 1073 1102 1194 1246 1153 69.51 6.0%

Synonyms

Semaphorin-6C; Semaphorin-Y; Sema Y

Gene Name

Sema6c

Gene ID

20360

UniProt

Q9WTM3

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A26-P605

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 95 439 1073 104 465 1124 Standard deviation 4.56 18.88 43.99 6.86 26.97 85.42 CV (%) 4.8% 4.3% 4.1% 6.6% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 95 439 1073 104 465 1124 Standard deviation 4.56 18.88 43.99 6.86 26.97 85.42 CV (%) 4.8% 4.3% 4.1% 6.6% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 95 439 1073 104 465 1124 Mean (pg/ml) 95 439 1073 104 465 1124 Standard deviation 4.56 18.88 43.99 6.86 26.97 85.42 Standard deviation 4.56 18.88 43.99 6.86 26.97 85.42 CV (%) 4.8% 4.3% 4.1% 6.6% 5.8% 7.6% CV (%) 4.8% 4.3% 4.1% 6.6% 5.8% 7.6%

Sensitivity

<50 pg/mL

Function

May be a stop signal for the dorsal root ganglion neurons in their target areas, and possibly also for other neurons. May also be involved in the maintenance and remodeling of neuronal connections.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse SEMA6C

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6C

Subcellular Location

Cell membrane. Single-pass type I membrane protein.

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.052 0.164 0.223 0.316 0.552 0.938 1.413 2.174 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.052 0.164 0.223 0.316 0.552 0.938 1.413 2.174 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.052 0.164 0.223 0.316 0.552 0.938 1.413 2.174 O.D. 0.052 0.164 0.223 0.316 0.552 0.938 1.413 2.174

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.