Rat SEMA6A/Semaphorin-6A ELISA Kit PicoKine®
Rat SEMA6A ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Sema6a in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Product Specifications
Background
Semaphorin-6A is a protein that in humans is encoded by the SEMA6A gene. This gene is mapped to 18q11. Semaphorin 6A is a transmembrane protein belonging to the semaphorin family. It functions as an axon guidance signal.
Reproducibility
Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 88 83 88 77 84 4.52 5.3% Sample 2 461 388 441 444 433 27.35 6.3% Sample 3 1323 1468 1499 1453 1435 67.17 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 88 83 88 77 84 4.52 5.3% Sample 1 88 83 88 77 84 4.52 5.3% Sample 2 461 388 441 444 433 27.35 6.3% Sample 2 461 388 441 444 433 27.35 6.3% Sample 3 1323 1468 1499 1453 1435 67.17 4.6% Sample 3 1323 1468 1499 1453 1435 67.17 4.6%
Synonyms
Semaphorin 6A; Sema6a
Gene Name
Sema6a
Gene ID
361324
UniProt
D3ZAG0
Reactivity
Rat
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Immunogen
Expression system for standard: NS0; Immunogen sequence: G19-T649
Capture Antibody
A monoclonal antibody from mouse
Detection Antibody
A biotinylated polyclonal antibody from goat
Applications
ELISA
Standard
10 ng/Tube
Sample Type
Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
Detection Range
62.5 pg/mL - 4,000 pg/mL
Precision
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 88 461 1323 91 471 1482 Standard deviation 3.52 26.28 60.86 4.64 32.50 103.74 CV (%) 4.0% 5.7% 4.6% 5.1% 6.9% 7.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 88 461 1323 91 471 1482 Standard deviation 3.52 26.28 60.86 4.64 32.50 103.74 CV (%) 4.0% 5.7% 4.6% 5.1% 6.9% 7.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 88 461 1323 91 471 1482 Mean (pg/ml) 88 461 1323 91 471 1482 Standard deviation 3.52 26.28 60.86 4.64 32.50 103.74 Standard deviation 3.52 26.28 60.86 4.64 32.50 103.74 CV (%) 4.0% 5.7% 4.6% 5.1% 6.9% 7.0% CV (%) 4.0% 5.7% 4.6% 5.1% 6.9% 7.0%
Sensitivity
<50 pg/mL
Components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Specificity
Natural and recombinant rat SEMA6A
Anticoagulant
Heparin, EDTA or citrate
Gene Name Synonym
Semaphorin 6A
Subcellular Location
Extracellular space. Integral component of plasma membrane.
Sample Data
Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.02 0.054 0.08 0.139 0.25 0.443 0.906 1.724 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.02 0.054 0.08 0.139 0.25 0.443 0.906 1.724 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.02 0.054 0.08 0.139 0.25 0.443 0.906 1.724 O.D. 0.02 0.054 0.08 0.139 0.25 0.443 0.906 1.724
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
BNC402395-500 1x 500 µL
CD25/ IL2RA (Activated Lymphocyte Marker) (IL2RA/2395), CF640R conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC942562-100 1x 100 µL
CD63 (Late Endosomes Marker) (rMX-49.129.5), CF594 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC472026-100 1x 100 µL
CD45 / LCA (Leucocyte Marker) (PTPRC/1783R + PTPRC/1975R), CF647 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC741815-500 1x 500 µL
Chromogranin A / CHGA (Neuroendocrine Marker) (CHGA/1815R), CF740 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 