Mouse SEMA6A/Semaphorin-6A ELISA Kit PicoKine®

Mouse SEMA6A ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Sema6a in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Semaphorin-6A is a protein that in humans is encoded by the SEMA6A gene. This gene is mapped to 18; 18 C. Semaphorin 6A is a transmembrane protein belonging to the semaphorin family. It functions as an axon guidance signal.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 101 94 96 98 97 2.58 2.6% Sample 2 465 451 513 472 475 23.06 4.8% Sample 3 1684 1888 1678 1813 1765 88.82 5.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 101 94 96 98 97 2.58 2.6% Sample 1 101 94 96 98 97 2.58 2.6% Sample 2 465 451 513 472 475 23.06 4.8% Sample 2 465 451 513 472 475 23.06 4.8% Sample 3 1684 1888 1678 1813 1765 88.82 5.0% Sample 3 1684 1888 1678 1813 1765 88.82 5.0%

Synonyms

Semaphorin-6A; Semaphorin Q; Sema Q; Semaphorin VIA; Sema VIA; Semaphorin-6A-1; SEMA6A-1

Gene Name

Sema6a

Gene ID

20358

UniProt

O35464

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G19-T649

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Particularly high levels in spinal cord, cerebellum, metencephalon, superior and inferior colliculus, diencephalon, olfactory bulb and eye.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 101 465 1684 113 475 1739 Standard deviation 5.96 20.00 69.04 7.46 27.08 135.64 CV (%) 5.9% 4.3% 4.1% 6.6% 5.7% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 101 465 1684 113 475 1739 Standard deviation 5.96 20.00 69.04 7.46 27.08 135.64 CV (%) 5.9% 4.3% 4.1% 6.6% 5.7% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 101 465 1684 113 475 1739 Mean (pg/ml) 101 465 1684 113 475 1739 Standard deviation 5.96 20.00 69.04 7.46 27.08 135.64 Standard deviation 5.96 20.00 69.04 7.46 27.08 135.64 CV (%) 5.9% 4.3% 4.1% 6.6% 5.7% 7.8% CV (%) 5.9% 4.3% 4.1% 6.6% 5.7% 7.8%

Sensitivity

<50 pg/mL

Function

Cell surface receptor for PLXNA2 that plays an important role in cell-cell signaling. Required for normal granule cell migration in the developing cerebellum. Promotes reorganization of the actin cytoskeleton and plays an important role in axon guidance in the developing central nervous system. Can act as repulsive axon guidance cue. Has repulsive action towards migrating granular neurons. May play a role in channeling sympathetic axons into the sympathetic chains and controlling the temporal sequence of sympathetic target innervation.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse SEMA6A

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A

Subcellular Location

Cell membrane. Single-pass type I membrane protein.

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.032 0.18 0.294 0.501 0.851 1.32 1.925 2.213 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.032 0.18 0.294 0.501 0.851 1.32 1.925 2.213 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.032 0.18 0.294 0.501 0.851 1.32 1.925 2.213 O.D. 0.032 0.18 0.294 0.501 0.851 1.32 1.925 2.213

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.