Rat SEMA4G ELISA Kit PicoKine®

Semaphorin-4G is a protein that in humans is encoded by the SEMA4G gene. This gene is mapped to 1q54. Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphoring (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. This gene encodes a class 4 semaphorin. This gene and the gene for mitochondrial ribosomal protein L43 overlap at map location 10q24.31 and are transcribed in opposite ions.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 44 51 58 60 53 3.57 6.7% Sample 2 95 90 88 85 90 4.3 4.8% Sample 3 133 136 145 158 143 10.3 7.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 44 51 58 60 53 3.57 6.7% Sample 1 44 51 58 60 53 3.57 6.7% Sample 2 95 90 88 85 90 4.3 4.8% Sample 2 95 90 88 85 90 4.3 4.8% Sample 3 133 136 145 158 143 10.3 7.2% Sample 3 133 136 145 158 143 10.3 7.2%

Sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (Semaphorin) 4G (Predicted) ; Semaphorin 4G; Sema4g; Sema4g_predicted; rCG_57705

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 44 95 133 34 91 140 Standard deviation 1.89 4.94 6.12 2.07 5.37 10.36 CV (%) 4.3% 5.2% 4.6% 6.1% 5.9% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 44 95 133 34 91 140 Standard deviation 1.89 4.94 6.12 2.07 5.37 10.36 CV (%) 4.3% 5.2% 4.6% 6.1% 5.9% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 44 95 133 34 91 140 Mean (pg/ml) 44 95 133 34 91 140 Standard deviation 1.89 4.94 6.12 2.07 5.37 10.36 Standard deviation 1.89 4.94 6.12 2.07 5.37 10.36 CV (%) 4.3% 5.2% 4.6% 6.1% 5.9% 7.4% CV (%) 4.3% 5.2% 4.6% 6.1% 5.9% 7.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.032 0.175 0.294 0.511 0.851 1.32 1.825 2.213 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.032 0.175 0.294 0.511 0.851 1.32 1.825 2.213 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.032 0.175 0.294 0.511 0.851 1.32 1.825 2.213 O.D. 0.032 0.175 0.294 0.511 0.851 1.32 1.825 2.213

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.