Mouse SEMA4G ELISA Kit PicoKine®

Mouse SEMA4G ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Sema4g in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Semaphorin-4G is a protein that in humans is encoded by the SEMA4G gene. This gene is mapped to 19; 19 C3. Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphoring (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. This gene encodes a class 4 semaphorin. This gene and the gene for mitochondrial ribosomal protein L43 overlap at map location 10q24.31 and are transcribed in opposite ions.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 44 51 58 60 53 2.29 4.3% Sample 2 187 174 190 188 185 11.09 6.0% Sample 3 503 513 506 522 511 32.7 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 44 51 58 60 53 2.29 4.3% Sample 1 44 51 58 60 53 2.29 4.3% Sample 2 187 174 190 188 185 11.09 6.0% Sample 2 187 174 190 188 185 11.09 6.0% Sample 3 503 513 506 522 511 32.7 6.4% Sample 3 503 513 506 522 511 32.7 6.4%

Synonyms

Semaphorin-4G; Sema4g

Gene Name

Sema4g

Gene ID

26456

UniProt

Q9WUH7

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: V18-M673

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Brain, spinal cord, and several sensory organs as well as specific populations of projection neurons.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 44 187 503 49 175 512 Standard deviation 2.16 8.04 20.62 3.23 10.15 38.91 CV (%) 4.9% 4.3% 4.1% 6.6% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 44 187 503 49 175 512 Standard deviation 2.16 8.04 20.62 3.23 10.15 38.91 CV (%) 4.9% 4.3% 4.1% 6.6% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 44 187 503 49 175 512 Mean (pg/ml) 44 187 503 49 175 512 Standard deviation 2.16 8.04 20.62 3.23 10.15 38.91 Standard deviation 2.16 8.04 20.62 3.23 10.15 38.91 CV (%) 4.9% 4.3% 4.1% 6.6% 5.8% 7.6% CV (%) 4.9% 4.3% 4.1% 6.6% 5.8% 7.6%

Sensitivity

<10 pg/mL

Function

Cell surface receptor for PLXNB2. May play a role in axon guidance.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse SEMA4G

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G

Subcellular Location

Cell membrane. Single-pass type I membrane protein.

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.055 0.113 0.17 0.283 0.49 0.877 1.549 2.315 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.055 0.113 0.17 0.283 0.49 0.877 1.549 2.315 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.055 0.113 0.17 0.283 0.49 0.877 1.549 2.315 O.D. 0.055 0.113 0.17 0.283 0.49 0.877 1.549 2.315

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.