Mouse CD69 ELISA Kit PicoKine®

Mouse CD69 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Cd69 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD69 (Cluster of Differentiation 69) is a human transmembrane C-Type lectin protein encoded by the CD69 gene. This gene is mapped to 12p13.31. This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 24 27 26 25 25 1.11 4.4% Sample 2 63 62 64 64 63 0.82 1.3% Sample 3 287 292 263 285 281 11.12 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 24 27 26 25 25 1.11 4.4% Sample 1 24 27 26 25 25 1.11 4.4% Sample 2 63 62 64 64 63 0.82 1.3% Sample 2 63 62 64 64 63 0.82 1.3% Sample 3 287 292 263 285 281 11.12 3.9% Sample 3 287 292 263 285 281 11.12 3.9%

Synonyms

Early activation antigen CD69; CD69; Cd69

Gene Name

Cd69

Gene ID

12515

UniProt

P37217

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: N62-R199

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils and platelets.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 24 63 287 27 84 429 Standard deviation 1.08 3.34 13.78 1.65 5.38 31.32 CV (%) 4.5% 5.3% 4.8% 6.1% 6.4% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 24 63 287 27 84 429 Standard deviation 1.08 3.34 13.78 1.65 5.38 31.32 CV (%) 4.5% 5.3% 4.8% 6.1% 6.4% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 24 63 287 27 84 429 Mean (pg/ml) 24 63 287 27 84 429 Standard deviation 1.08 3.34 13.78 1.65 5.38 31.32 Standard deviation 1.08 3.34 13.78 1.65 5.38 31.32 CV (%) 4.5% 5.3% 4.8% 6.1% 6.4% 7.3% CV (%) 4.5% 5.3% 4.8% 6.1% 6.4% 7.3%

Sensitivity

<20 pg/mL

Function

Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse CD69

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

CD69 antigen

Subcellular Location

Membrane. Single-pass type II membrane protein.

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.051 0.108 0.203 0.377 0.672 1.188 1.84 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.051 0.108 0.203 0.377 0.672 1.188 1.84 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.051 0.108 0.203 0.377 0.672 1.188 1.84 O.D. 0.008 0.051 0.108 0.203 0.377 0.672 1.188 1.84

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.