Human CD69 ELISA Kit PicoKine®

Human CD69 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CD69 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD69 (Cluster of Differentiation 69) is a human transmembrane C-Type lectin protein encoded by the CD69 gene. This gene is mapped to 6 F3; 6 63.21 cM. This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 109 106 115 107 109 3.49 3.2% Sample 2 470 422 410 478 445 29.44 6.6% Sample 3 1804 1954 1992 1834 1896 83.42 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 109 106 115 107 109 3.49 3.2% Sample 1 109 106 115 107 109 3.49 3.2% Sample 2 470 422 410 478 445 29.44 6.6% Sample 2 470 422 410 478 445 29.44 6.6% Sample 3 1804 1954 1992 1834 1896 83.42 4.4% Sample 3 1804 1954 1992 1834 1896 83.42 4.4%

Synonyms

Early activation antigen CD69; Activation inducer molecule; AIM; BL-AC/P26; C-type lectin domain family 2 member C; EA1; Early T-cell activation antigen p60; GP32/28; Leukocyte surface antigen Leu-23; MLR-3; CD69; CLEC2C

Gene Name

CD69

Gene ID

969

UniProt

Q07108

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S62-K199

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 109 470 1804 112 481 1927 Standard deviation 5.23 23.03 82.98 6.27 33.19 123.33 CV (%) 4.8% 4.9% 4.6% 5.6% 6.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 109 470 1804 112 481 1927 Standard deviation 5.23 23.03 82.98 6.27 33.19 123.33 CV (%) 4.8% 4.9% 4.6% 5.6% 6.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 109 470 1804 112 481 1927 Mean (pg/ml) 109 470 1804 112 481 1927 Standard deviation 5.23 23.03 82.98 6.27 33.19 123.33 Standard deviation 5.23 23.03 82.98 6.27 33.19 123.33 CV (%) 4.8% 4.9% 4.6% 5.6% 6.9% 6.4% CV (%) 4.8% 4.9% 4.6% 5.6% 6.9% 6.4%

Sensitivity

<10 pg/mL

Function

Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CD69

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

CD69 molecule

Subcellular Location

Membrane. Single-pass type II membrane protein.

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.057 0.106 0.156 0.256 0.456 0.856 1.592 2.756 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.057 0.106 0.156 0.256 0.456 0.856 1.592 2.756 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.057 0.106 0.156 0.256 0.456 0.856 1.592 2.756 O.D. 0.057 0.106 0.156 0.256 0.456 0.856 1.592 2.756

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.