Human CLEC14A ELISA Kit PicoKine®

CLEC14A (C-type lectin domain family 14 member A; also EGFR5) is a 51 kDa (predicted) member of the C-type lectin domain family of proteins. This gene is mapped to 14q21.1. This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. This family member plays a role in cell-cell adhesion and angiogenesis. It functions in filopodia formation, cell migration and tube formation. Due to its presence at higher levels in tumor endothelium than in normal tissue endothelium, it is considered to be a candidate for tumor vascular targeting.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 247 237 237 234 238 4.91 2.0% Sample 2 1236 1184 1263 1230 1228 28.41 2.3% Sample 3 4806 4354 5047 4709 4729 249.03 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 247 237 237 234 238 4.91 2.0% Sample 1 247 237 237 234 238 4.91 2.0% Sample 2 1236 1184 1263 1230 1228 28.41 2.3% Sample 2 1236 1184 1263 1230 1228 28.41 2.3% Sample 3 4806 4354 5047 4709 4729 249.03 5.2% Sample 3 4806 4354 5047 4709 4729 249.03 5.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 247 1236 4806 295 1245 4921 Standard deviation 11.12 70.45 221.08 20.95 85.91 314.94 CV (%) 4.5% 5.7% 4.6% 7.1% 6.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 247 1236 4806 295 1245 4921 Standard deviation 11.12 70.45 221.08 20.95 85.91 314.94 CV (%) 4.5% 5.7% 4.6% 7.1% 6.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 247 1236 4806 295 1245 4921 Mean (pg/ml) 247 1236 4806 295 1245 4921 Standard deviation 11.12 70.45 221.08 20.95 85.91 314.94 Standard deviation 11.12 70.45 221.08 20.95 85.91 314.94 CV (%) 4.5% 5.7% 4.6% 7.1% 6.9% 6.4% CV (%) 4.5% 5.7% 4.6% 7.1% 6.9% 6.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.101 0.156 0.256 0.456 0.896 1.592 2.256 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.101 0.156 0.256 0.456 0.896 1.592 2.256 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.101 0.156 0.256 0.456 0.896 1.592 2.256 O.D. 0.057 0.101 0.156 0.256 0.456 0.896 1.592 2.256

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.