Mouse CD206/CLEC13D ELISA Kit PicoKine®

The mannose receptor (Cluster of Differentiation 206, CD206) is a C-type lectin primarily present on the surface of macrophages and immature dendritic cells, but is also expressed on the surface of skin cells such as human dermal fibroblasts and keratinocytes. It is the first member of a family of endocytic receptors that includes Endo180 (CD280), M-type PLA2R, and DEC-205 (CD205) . This gene is mapped to 2 A2; 2 10.46 cM. The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1150 1144 1176 1080 1137 35.30 3.1% Sample 2 6191 5982 6880 6356 6352 332.27 5.2% Sample 3 22279 22618 21731 19374 21500 1267.87 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1150 1144 1176 1080 1137 35.30 3.1% Sample 1 1150 1144 1176 1080 1137 35.30 3.1% Sample 2 6191 5982 6880 6356 6352 332.27 5.2% Sample 2 6191 5982 6880 6356 6352 332.27 5.2% Sample 3 22279 22618 21731 19374 21500 1267.87 5.8% Sample 3 22279 22618 21731 19374 21500 1267.87 5.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1150 6191 22279 1234 6199 23488 Standard deviation 59.80 266.21 913.44 81.44 359.54 1550.21 CV (%) 5.2% 4.3% 4.1% 6.6% 5.8% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1150 6191 22279 1234 6199 23488 Standard deviation 59.80 266.21 913.44 81.44 359.54 1550.21 CV (%) 5.2% 4.3% 4.1% 6.6% 5.8% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1150 6191 22279 1234 6199 23488 Mean (pg/ml) 1150 6191 22279 1234 6199 23488 Standard deviation 59.80 266.21 913.44 81.44 359.54 1550.21 Standard deviation 59.80 266.21 913.44 81.44 359.54 1550.21 CV (%) 5.2% 4.3% 4.1% 6.6% 5.8% 6.6% CV (%) 5.2% 4.3% 4.1% 6.6% 5.8% 6.6%

Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.02 0.065 0.123 0.214 0.394 0.68 1.252 2.186 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.02 0.065 0.123 0.214 0.394 0.68 1.252 2.186 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.02 0.065 0.123 0.214 0.394 0.68 1.252 2.186 O.D. 0.02 0.065 0.123 0.214 0.394 0.68 1.252 2.186

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.