Human CLEC10A ELISA Kit PicoKine®

C-type lectin domain family 10 member A also known as CLEC10A is a protein that in humans is encoded by the CLEC10A gene. This gene is mapped to 17p13.1. This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may function as a cell surface antigen. Two transcript variants encoding distinct isoforms have been identified for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 38 33 32 34 2.27 6.6% Sample 2 244 232 243 247 241 5.67 2.3% Sample 3 404 404 433 399 410 13.43 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 38 33 32 34 2.27 6.6% Sample 1 34 38 33 32 34 2.27 6.6% Sample 2 244 232 243 247 241 5.67 2.3% Sample 2 244 232 243 247 241 5.67 2.3% Sample 3 404 404 433 399 410 13.43 3.2% Sample 3 404 404 433 399 410 13.43 3.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 244 404 37 249 456 Standard deviation 1.87 12.4 17.38 2.26 16.4 34.2 CV (%) 5.5% 5.1% 4.3% 6.1% 6.6% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 244 404 37 249 456 Standard deviation 1.87 12.4 17.38 2.26 16.4 34.2 CV (%) 5.5% 5.1% 4.3% 6.1% 6.6% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 34 244 404 37 249 456 Mean (pg/ml) 34 244 404 37 249 456 Standard deviation 1.87 12.4 17.38 2.26 16.4 34.2 Standard deviation 1.87 12.4 17.38 2.26 16.4 34.2 CV (%) 5.5% 5.1% 4.3% 6.1% 6.6% 7.5% CV (%) 5.5% 5.1% 4.3% 6.1% 6.6% 7.5%

Probable role in regulating adaptive and innate immune responses. Binds in a calcium-dependent manner to terminal galactose and N-acetylgalactosamine units, linked to serine or threonine. These sugar moieties are known as Tn-Ag and are expressed in a variety of carcinoma cells.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.011 0.061 0.119 0.207 0.401 0.742 1.481 2.259 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.011 0.061 0.119 0.207 0.401 0.742 1.481 2.259 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.011 0.061 0.119 0.207 0.401 0.742 1.481 2.259 O.D. 0.011 0.061 0.119 0.207 0.401 0.742 1.481 2.259

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.