Human CLEC4C ELISA Kit PicoKine®

CLEC4C is a membrane protein of plasmacytoid dendritic cells used as a marker for this kind of cells and denoted as CD303 in the nomenclature of the Cluster of differentiation. This gene is mapped to 12p13.31. This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may play a role in dendritic cell function. Two transcript variants encoding distinct isoforms have been identified for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 23 22 20 22 21 1.08 5.1% Sample 2 84 91 89 83 86 3.34 3.8% Sample 3 465 429 503 512 477 32.97 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 23 22 20 22 21 1.08 5.1% Sample 1 23 22 20 22 21 1.08 5.1% Sample 2 84 91 89 83 86 3.34 3.8% Sample 2 84 91 89 83 86 3.34 3.8% Sample 3 465 429 503 512 477 32.97 6.9% Sample 3 465 429 503 512 477 32.97 6.9%

C-type lectin domain family 4 member C; Blood dendritic cell antigen 2; BDCA-2; C-type lectin superfamily member 7; Dendritic lectin; CD303; CLEC4C; BDCA2; CLECSF11; CLECSF7; DLEC; HECL; UNQ9361; PRO34150

Expressed in plasmacytoid dendritic cells (PDCs) . Constitutively expressed in immature monocyte-derived dendritic cells (iMDDC) and is significantly down-regulated upon maturation with LPS but not with TNF-alpha.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 23 84 465 28 117 482 Standard deviation 1.13 3.61 19.07 1.90 6.79 36.63 CV (%) 4.9% 4.3% 4.1% 6.8% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 23 84 465 28 117 482 Standard deviation 1.13 3.61 19.07 1.90 6.79 36.63 CV (%) 4.9% 4.3% 4.1% 6.8% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 23 84 465 28 117 482 Mean (pg/ml) 23 84 465 28 117 482 Standard deviation 1.13 3.61 19.07 1.90 6.79 36.63 Standard deviation 1.13 3.61 19.07 1.90 6.79 36.63 CV (%) 4.9% 4.3% 4.1% 6.8% 5.8% 7.6% CV (%) 4.9% 4.3% 4.1% 6.8% 5.8% 7.6%

Lectin-type cell surface receptor which may play a role in antigen capturing by dendritic cells. Specifically recognizes non-sialylated galactose-terminated biantennary glycans containing the trisaccharide epitope Gal (beta1-3/4) GlcNAc (beta1-2) Man. Binds to serum IgG. Efficiently targets ligand into antigen-processing and peptide-loading compartments for presentation to T-cells. May mediate potent inhibition of induction of IFN-alpha/beta expression in plasmacytoid dendritic cells. May act as a signaling receptor that activates protein-tyrosine kinases and mobilizes intracellular calcium.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.004 0.122 0.207 0.365 0.602 0.973 1.584 2.166 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.004 0.122 0.207 0.365 0.602 0.973 1.584 2.166 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.004 0.122 0.207 0.365 0.602 0.973 1.584 2.166 O.D. 0.004 0.122 0.207 0.365 0.602 0.973 1.584 2.166

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.