Mouse GAS1 ELISA Kit PicoKine®

Mouse GAS1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Gas1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Growth arrest-specific protein 1 is a protein that in humans is encoded by the GAS1 gene. This gene is mapped to 13 B2; 13 31.92 cM. Growth arrest-specific 1 plays a role in growth suppression. GAS1 blocks entry to S phase and prevents cycling of normal and transfor

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 573 536 539 600 562 26.31 4.6% Sample 2 1930 1980 1951 1871 1933 39.95 2.0% Sample 3 8898 10038 9685 9879 9625 437.95 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 573 536 539 600 562 26.31 4.6% Sample 1 573 536 539 600 562 26.31 4.6% Sample 2 1930 1980 1951 1871 1933 39.95 2.0% Sample 2 1930 1980 1951 1871 1933 39.95 2.0% Sample 3 8898 10038 9685 9879 9625 437.95 4.5% Sample 3 8898 10038 9685 9879 9625 437.95 4.5%

Synonyms

Growth arrest-specific protein 1; GAS-1; Gas1; Gas-1

Gene Name

Gas1

Gene ID

14451

UniProt

Q01721

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L39-S315

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 573 1930 8898 591 2046 9517 Standard deviation 23.49 110.01 409.31 34.28 141.17 609.09 CV (%) 4.1% 5.7% 4.6% 5.8% 6.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 573 1930 8898 591 2046 9517 Standard deviation 23.49 110.01 409.31 34.28 141.17 609.09 CV (%) 4.1% 5.7% 4.6% 5.8% 6.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 573 1930 8898 591 2046 9517 Mean (pg/ml) 573 1930 8898 591 2046 9517 Standard deviation 23.49 110.01 409.31 34.28 141.17 609.09 Standard deviation 23.49 110.01 409.31 34.28 141.17 609.09 CV (%) 4.1% 5.7% 4.6% 5.8% 6.9% 6.4% CV (%) 4.1% 5.7% 4.6% 5.8% 6.9% 6.4%

Sensitivity

<50 pg/mL

Function

Specific growth arrest protein involved in growth suppression. Blocks entry to S phase. Prevents cycling of normal and transformed cells.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse GAS1

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Growth arrest specific 1

Subcellular Location

Cell membrane. GPI-anchor.

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.045 0.137 0.182 0.295 0.496 0.897 1.463 2.025 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.045 0.137 0.182 0.295 0.496 0.897 1.463 2.025 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.045 0.137 0.182 0.295 0.496 0.897 1.463 2.025 O.D. 0.045 0.137 0.182 0.295 0.496 0.897 1.463 2.025

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.