Human GAS1 ELISA Kit PicoKine®

Human GAS1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human GAS1 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Growth arrest-specific protein 1 is a protein that in humans is encoded by the GAS1 gene. This gene is mapped to 9q21.33. Growth arrest-specific 1 plays a role in growth suppression. GAS1 blocks entry to S phase and prevents cycling of normal and transformed cells. Gas1 is a putative tumor suppressor gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 53 50 52 52 1.80 3.4% Sample 2 228 261 228 264 245 17.28 7.0% Sample 3 845 788 838 845 829 23.84 2.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 53 50 52 52 1.80 3.4% Sample 1 55 53 50 52 52 1.80 3.4% Sample 2 228 261 228 264 245 17.28 7.0% Sample 2 228 261 228 264 245 17.28 7.0% Sample 3 845 788 838 845 829 23.84 2.8% Sample 3 845 788 838 845 829 23.84 2.8%

Synonyms

Growth arrest-specific protein 1; GAS-1; GAS1

Gene Name

GAS1

Gene ID

2619

UniProt

P54826

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L40-S318

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

2 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 55 228 845 60 239 884 Standard deviation 2.31 14.36 38.03 3.18 15.30 63.65 CV (%) 4.2% 6.3% 4.5% 5.3% 6.4% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 55 228 845 60 239 884 Standard deviation 2.31 14.36 38.03 3.18 15.30 63.65 CV (%) 4.2% 6.3% 4.5% 5.3% 6.4% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 55 228 845 60 239 884 Mean (pg/ml) 55 228 845 60 239 884 Standard deviation 2.31 14.36 38.03 3.18 15.30 63.65 Standard deviation 2.31 14.36 38.03 3.18 15.30 63.65 CV (%) 4.2% 6.3% 4.5% 5.3% 6.4% 7.2% CV (%) 4.2% 6.3% 4.5% 5.3% 6.4% 7.2%

Sensitivity

<10 pg/mL

Function

Specific growth arrest protein involved in growth suppression. Blocks entry to S phase. Prevents cycling of normal and transformed cells.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human GAS1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Growth arrest specific 1

Subcellular Location

Cell membrane. GPI-anchor.

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.004 0.114 0.189 0.339 0.581 1.001 1.707 2.835 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.004 0.114 0.189 0.339 0.581 1.001 1.707 2.835 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.004 0.114 0.189 0.339 0.581 1.001 1.707 2.835 O.D. 0.004 0.114 0.189 0.339 0.581 1.001 1.707 2.835

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.