Human SPINK5 ELISA Kit PicoKine®

Lympho-epithelial Kazal-type-related inhibitor (LEKTI) also known as serine protease inhibitor Kazal-type 5 (SPINK5) is a protein that in humans is encoded by the SPINK5 gene. This gene is mapped to 5q32. This gene encodes a multidomain serine protease inhibitor that contains 15 potential inhibitory domains. The encoded preproprotein is proteolytically processed to generate multiple protein products, which may exhibit unique activities and specificities. These proteins may play a role in skin and hair morphogenesis, as well as anti-inflammatory and antimicrobial protection of mucous epithelia. Mutations in this gene may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy. This gene is present in a gene cluster on chromosome 5. Alternative splicing results in multiple transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 436 480 445 428 447 19.84 4.4% Sample 2 1531 1538 1402 1495 1491 54.18 3.6% Sample 3 7423 8539 7844 7400 7801 461.02 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 436 480 445 428 447 19.84 4.4% Sample 1 436 480 445 428 447 19.84 4.4% Sample 2 1531 1538 1402 1495 1491 54.18 3.6% Sample 2 1531 1538 1402 1495 1491 54.18 3.6% Sample 3 7423 8539 7844 7400 7801 461.02 5.9% Sample 3 7423 8539 7844 7400 7801 461.02 5.9%

Highly expressed in the thymus and stratum corneum. Also found in the oral mucosa, parathyroid gland, Bartholin's glands, tonsils, and vaginal epithelium. Very low levels are detected in lung, kidney, and prostate.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 436 1531 7423 537 2009 8417 Standard deviation 20.93 87.27 341.46 27.39 118.53 538.69 CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 436 1531 7423 537 2009 8417 Standard deviation 20.93 87.27 341.46 27.39 118.53 538.69 CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 436 1531 7423 537 2009 8417 Mean (pg/ml) 436 1531 7423 537 2009 8417 Standard deviation 20.93 87.27 341.46 27.39 118.53 538.69 Standard deviation 20.93 87.27 341.46 27.39 118.53 538.69 CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4% CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4%

Serine protease inhibitor, probably important for the anti-inflammatory and/or antimicrobial protection of mucous epithelia. Contribute to the integrity and protective barrier function of the skin by regulating the activity of defense-activating and desquamation-involved proteases. Inhibits KLK5, it's major target, in a pH-dependent manner. Inhibits KLK7, KLK14 CASP14, and trypsin.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.008 0.087 0.171 0.295 0.574 1.1 1.981 2.761 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.008 0.087 0.171 0.295 0.574 1.1 1.981 2.761 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.008 0.087 0.171 0.295 0.574 1.1 1.981 2.761 O.D. 0.008 0.087 0.171 0.295 0.574 1.1 1.981 2.761

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.