Human ULBP3 ELISA Kit PicoKine®

UL16 binding protein 3 (ULBP3) is a cell surface glycoprotein encoded by ULBP3 gene located on the chromosome 6. It is mapped to 6q25.1. ULBP3 is related to MHC class I molecules, but its gene maps outside the MHC locus. The domain structure of ULBP3 differs significantly from those of conventional MHC class I molecules. It does not contain the α3 domain and the transmembrane segment. ULBP3 is thus composed of only the α1α2 domain which is linked to the cell membrane by the GPI anchor. It functions as a stress-induced ligand for NKG2D receptor. The protein encoded by this gene is one of several related ligands of the KLRK1/NKG2D receptor, which is found in primary NK cells. Binding of these ligands to the receptor activates several signal transduction pathways, including the JAK2, STAT5, and ERK pathways. The encoded protein is expressed solubly and on the surface of many tumor cells, making it potentially an important target for therapeutics.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 98 98 102 85 95 6.41 6.7% Sample 2 477 459 496 467 474 13.82 2.9% Sample 3 1133 1050 1116 1070 1092 33.55 3.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 98 98 102 85 95 6.41 6.7% Sample 1 98 98 102 85 95 6.41 6.7% Sample 2 477 459 496 467 474 13.82 2.9% Sample 2 477 459 496 467 474 13.82 2.9% Sample 3 1133 1050 1116 1070 1092 33.55 3.0% Sample 3 1133 1050 1116 1070 1092 33.55 3.0%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 98 477 1133 101 482 1425 Standard deviation 3.92 20.51 52.12 5.15 26.99 84.08 CV (%) 4.0% 4.3% 4.6% 5.1% 5.6% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 98 477 1133 101 482 1425 Standard deviation 3.92 20.51 52.12 5.15 26.99 84.08 CV (%) 4.0% 4.3% 4.6% 5.1% 5.6% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 98 477 1133 101 482 1425 Mean (pg/ml) 98 477 1133 101 482 1425 Standard deviation 3.92 20.51 52.12 5.15 26.99 84.08 Standard deviation 3.92 20.51 52.12 5.15 26.99 84.08 CV (%) 4.0% 4.3% 4.6% 5.1% 5.6% 5.9% CV (%) 4.0% 4.3% 4.6% 5.1% 5.6% 5.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.017 0.062 0.098 0.169 0.299 0.578 1.016 1.805 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.017 0.062 0.098 0.169 0.299 0.578 1.016 1.805 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.017 0.062 0.098 0.169 0.299 0.578 1.016 1.805 O.D. 0.017 0.062 0.098 0.169 0.299 0.578 1.016 1.805

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.