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Mouse ENTPD2 ELISA Kit PicoKine®

Mouse ENTPD2 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Entpd2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Ectonucleoside triphosphate diphosphohydrolase 2 is an enzyme that in humans is encoded by the ENTPD2 gene. This gene is mapped to 2; 2 A3. The protein encoded by this gene is the type 2 enzyme of the ecto-nucleoside triphosphate diphosphohydrolase family (E-NTPDase) . E-NTPDases are a family of ecto-nucleosidases that hydrolyze 5'-triphosphates. This ecto-ATPase is an integral membrane protein. Alternative splicing of this gene results in multiple transcript variants. It has been shown, by scientists from the University of Warwick, that E-NTPDase2 stimulates the growth of the eye: by testing the enzyme on tadpoles, the tadpoles were found to develop extra eyes on their body.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 22 23 22 23 22 0.50 2.2% Sample 2 91 104 95 96 96 4.71 4.9% Sample 3 412 477 456 451 449 23.48 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 22 23 22 23 22 0.50 2.2% Sample 1 22 23 22 23 22 0.50 2.2% Sample 2 91 104 95 96 96 4.71 4.9% Sample 2 91 104 95 96 96 4.71 4.9% Sample 3 412 477 456 451 449 23.48 5.2% Sample 3 412 477 456 451 449 23.48 5.2%

Synonyms

Ectonucleoside triphosphate diphosphohydrolase 2; NTPDase 2; CD39 antigen-like 1; Ecto-ATP diphosphohydrolase 2; Ecto-ATPDase 2; Ecto-ATPase 2; Entpd2; Cd39l1

Gene Name

Entpd2

Gene ID

12496

UniProt

O55026

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: C26-S462

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Field of Research

Cancer, Metabolism, Neurology Process, Neuroscience

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 22 91 412 28 113 431 Standard deviation 1.06 4.73 22.25 1.43 6.22 25.86 CV (%) 4.8% 5.2% 5.4% 5.1% 5.5% 6.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 22 91 412 28 113 431 Standard deviation 1.06 4.73 22.25 1.43 6.22 25.86 CV (%) 4.8% 5.2% 5.4% 5.1% 5.5% 6.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 22 91 412 28 113 431 Mean (pg/ml) 22 91 412 28 113 431 Standard deviation 1.06 4.73 22.25 1.43 6.22 25.86 Standard deviation 1.06 4.73 22.25 1.43 6.22 25.86 CV (%) 4.8% 5.2% 5.4% 5.1% 5.5% 6.0% CV (%) 4.8% 5.2% 5.4% 5.1% 5.5% 6.0%

Sensitivity

<10 pg/mL

Function

In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Hydrolyzes ADP only to a marginal extent.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Product Datasheet

https://www.bosterbio.com/datasheet?sku=EK2014

Product MSDS

https://www.bosterbio.com/msds?sku=EK2014

Specificity

Natural and recombinant mouse ENTPD2

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Ectonucleoside triphosphate diphosphohydrolase 2

Subcellular Location

Cell membrane. Multi-pass membrane protein.

Protein Name

Ectonucleoside triphosphate diphosphohydrolase 2

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.095 0.172 0.23 0.343 0.534 0.851 1.374 2.098 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.095 0.172 0.23 0.343 0.534 0.851 1.374 2.098 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.095 0.172 0.23 0.343 0.534 0.851 1.374 2.098 O.D. 0.095 0.172 0.23 0.343 0.534 0.851 1.374 2.098

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
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